People

Dr Philip Reeves

Senior Lecturer
School of Life Sciences
Dr Philip Reeves

Profile

Biography

2005present. Lecturer, School of Biological Sciences, University of Essex, Colchester, UK. 1994-2005. Postdoctoral Research Fellow/Research Scientist, Department of Biology, MIT, Cambridge (USA). Laboratory of H. Gobind Khorana. 1991-1994. Postdoctoral Research Fellow, Department of Biological Sciences, University of Warwick, Coventry, UK. 1987-1991. PhD. in Molecular biology of protein secretion. University of Warwick.Coventry, UK.Supervisor George Salmond 1986-1987. Research Technician Department of Biological Sciences, University of Warwick, Coventry, UK. 1983-1986. BSc. (Hons) Microbiology and Microbial Technology. University of Warwick, Coventry, UK. Fellow of the Higher Education Authority (UK) Member of the Biochemical Society (UK) Member of the American Society for Biochemistry and Molecular Biology (USA) Research students: Mr Aidan Cooke- MPHD CELL AND MOLECULAR BIOLOGY Mr James Embling- MPHD BIOLOGICAL SCIENCES

Qualifications

  • BSc. (Upper 2nd class honours) Microbiology and Microbial Technology (Warwick)

  • PhD. Extracellular Enzyme Secretion in Erwinia (Warwick)

  • Post Graduate Certificate in Higher EducationPractice(Essex)

Research and professional activities

Research interests

Structural properties and activation mechanism of rhodopsin, the dim-light human photoreceptor (details)

Open to supervise

Biosynthesis and cellular targeting of G-protein coupled receptors (GPCRs)

Open to supervise

Development of gene expression systems for large-scale production and purification of membrane proteins (details)

Open to supervise

Molecular mechanisms of Retinitis Pigmentosa caused by inherited mutations in rhodopsin

Open to supervise

Current research

Molecular mechanism of vision losscaused byRetinitis Pigmentosa mutations in rhodopsin Funded by RP Fighting Blindness and Fight for Sight

Folding and activation mechanism(s) of the visual receptor rhodopsin. Funded by the National Institutes of Health (USA)

Teaching and supervision

Current teaching responsibilities

  • Transferable Skills in Life Sciences (BS143)

  • Enterprise and Employability for the Biosciences (BS211)

  • Biomedical Science: Practice and Employability (BS214)

  • Molecular Biology: Genes, Proteins and Disease (BS221)

  • Cell Signalling (BS327)

  • Mechanisms of Neurological Disease (BS350)

  • Research Project in Biomolecular Science (BS831)

Publications

Journal articles (59)

Athanasiou, D., Aguila, M., Bellingham, J., Li, W., McCulley, C., Reeves, PJ. and Cheetham, ME., (2018). The molecular and cellular basis of rhodopsin retinitis pigmentosa reveals potential strategies for therapy. Progress in Retinal and Eye Research. 62, 1-23

Athanasiou, D., Aguila, M., Opefi, CA., South, K., Bellingham, J., Bevilacqua, D., Munro, PM., Kanuga, N., Mackenzie, FE., Dubis, AM., Georgiadis, A., Graca, AB., Pearson, RA., Ali, RR., Sakami, S., Palczewski, K., Sherman, MY., Reeves, PJ. and Cheetham, ME., (2017). Rescue of mutant rhodopsin traffic by metformin-induced AMPK activation accelerates photoreceptor degeneration. Human Molecular Genetics. 26 (2), ddw387-ddw387

Sanchez-Reyes, OB., Cooke, ALG., Tranter, DB., Rashid, D., Eilers, M., Reeves, PJ. and Smith, SO., (2017). G Protein-Coupled Receptors Contain Two Conserved Packing Clusters. Biophysical Journal. 112 (11), 2315-2326

Kimata, N., Pope, A., Sanchez-Reyes, OB., Eilers, M., Opefi, CA., Ziliox, M., Reeves, PJ. and Smith, SO., (2016). Free backbone carbonyls mediate rhodopsin activation. Nature Structural & Molecular Biology. 23 (8), 738-743

Sandoval, A., Eichler, S., Madathil, S., Reeves, PJ., Fahmy, K. and Böckmann, RA., (2016). The Molecular Switching Mechanism at the Conserved D(E)RY Motif in Class-A GPCRs. Biophysical Journal. 111 (1), 79-89

Kimata, N., Pope, A., Eilers, M., Opefi, CA., Ziliox, M., Hirshfeld, A., Zaitseva, E., Vogel, R., Sheves, M., Reeves, PJ. and Smith, SO., (2016). Retinal orientation and interactions in rhodopsin reveal a two-stage trigger mechanism for activation. Nature Communications. 7 (1), 12683-

Kimata, N., Reeves, PJ. and Smith, SO., (2015). Uncovering the triggers for GPCR activation using solid-state NMR spectroscopy. Journal of Magnetic Resonance. 253, 111-118

Opefi, CA., Tranter, D., Smith, SO. and Reeves, PJ., (2015). Construction of Stable Mammalian Cell Lines for Inducible Expression of G Protein-Coupled Receptors. MEMBRANE PROTEINS - PRODUCTION AND FUNCTIONAL CHARACTERIZATION. 556, 283-305

Taddese, B., Upton, GJG., Bailey, GR., Jordan, SRD., Abdulla, NY., Reeves, PJ. and Reynolds, CA., (2014). Do Plants Contain G Protein-Coupled Receptors?. PLANT PHYSIOLOGY. 164 (1), 287-307

Chintapalli, SV., Illingworth, CJR., Upton, GJG., Sacquin-Mora, S., Reeves, PJ., Mohammedali, HS. and Reynolds, CA., (2014). Assessing the effect of dynamics on the closed-loop protein-folding hypothesis. Journal of The Royal Society Interface. 11 (91), 20130935-20130935

Pope, A., Eilers, M., Reeves, PJ. and Smith, SO., (2014). Amino acid conservation and interactions in rhodopsin: Probing receptor activation by NMR spectroscopy. Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1837 (5), 683-693

Vohra, S., Taddese, B., Conner, AC., Poyner, DR., Hay, DL., Barwell, J., Reeves, PJ., Upton, GJG. and Reynolds, CA., (2013). Similarity between class A and class B G-protein-coupled receptors exemplified through calcitonin gene-related peptide receptor modelling and mutagenesis studies. Journal of The Royal Society Interface. 10 (79), 20120846-20120846

Opefi, CA., South, K., Reynolds, CA., Smith, SO. and Reeves, PJ., (2013). Retinitis Pigmentosa Mutants Provide Insight into the Role of the N-terminal Cap in Rhodopsin Folding, Structure, and Function. Journal of Biological Chemistry. 288 (47), 33912-33926

Eilers, M., Goncalves, JA., Ahuja, S., Kirkup, C., Hirshfeld, A., Simmerling, C., Reeves, PJ., Sheves, M. and Smith, SO., (2012). Structural Transitions of Transmembrane Helix 6 in the Formation of Metarhodopsin I. The Journal of Physical Chemistry B. 116 (35), 10477-10489

Arakawa, M., Chakraborty, R., Upadhyaya, J., Eilers, M., Reeves, PJ., Smith, SO. and Chelikani, P., (2011). Structural and functional roles of small group-conserved amino acids present on helix-H7 in the β2-adrenergic receptor. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1808 (4), 1170-1178

Chintapalli, SV., Yew, BK., Illingworth, CJR., Upton, GJG., Reeves, PJ., Parkes, KEB., Snell, CR. and Reynolds, CA., (2010). Closed loop folding units from structural alignments: Experimental foldons revisited. Journal of Computational Chemistry. 31 (15), 2689-2701

Goncalves, JA., South, K., Ahuja, S., Zaitseva, E., Opefi, CA., Eilers, M., Vogel, R., Reeves, PJ. and Smith, SO., (2010). Highly conserved tyrosine stabilizes the active state of rhodopsin. Proceedings of the National Academy of Sciences. 107 (46), 19861-19866

Hornak, V., Ahuja, S., Eilers, M., Goncalves, JA., Sheves, M., Reeves, PJ. and Smith, SO., (2010). Light Activation of Rhodopsin: Insights from Molecular Dynamics Simulations Guided by Solid-State NMR Distance Restraints. Journal of Molecular Biology. 396 (3), 510-527

Ahuja, S., Crocker, E., Eilers, M., Hornak, V., Hirshfeld, A., Ziliox, M., Syrett, N., Reeves, PJ., Khorana, HG., Sheves, M. and Smith, SO., (2009). Location of the Retinal Chromophore in the Activated State of Rhodopsin*. Journal of Biological Chemistry. 284 (15), 10190-10201

Ahuja, S., Hornak, V., Yan, ECY., Syrett, N., Goncalves, JA., Hirshfeld, A., Ziliox, M., Sakmar, TP., Sheves, M., Reeves, PJ., Smith, SO. and Eilers, M., (2009). Helix movement is coupled to displacement of the second extracellular loop in rhodopsin activation. Nature Structural & Molecular Biology. 16 (2), 168-175

Takayama, H., Chelikani, P., Reeves, PJ., Zhang, S. and Khorana, HG., (2008). High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81. PLoS ONE. 3 (6), e2314-e2314

Vohra, S., Chintapalli, SV., Illingworth, CJR., Reeves, PJ., Mullineaux, PM., Clark, HSX., Dean, MK., Upton, GJG. and Reynolds, CA., (2007). Computational studies of Family A and Family B GPCRs. Biochemical Society Transactions. 35 (4), 749-754

Chelikani, P., Hornak, V., Eilers, M., Reeves, PJ., Smith, SO., RajBhandary, UL. and Khorana, HG., (2007). Role of group-conserved residues in the helical core of beta2-adrenergic receptor. Proceedings of the National Academy of Sciences. 104 (17), 7027-7032

McKibbin, C., Farmer, NA., Jeans, C., Reeves, PJ., Khorana, HG., Wallace, BA., Edwards, PC., Villa, C. and Booth, PJ., (2007). Opsin Stability and Folding: Modulation by Phospholipid Bicelles. Journal of Molecular Biology. 374 (5), 1319-1332

McKibbin, C., Toye, AM., Reeves, PJ., Khorana, HG., Edwards, PC., Villa, C. and Booth, PJ., (2007). Opsin Stability and Folding: The Role of Cys185 and Abnormal Disulfide Bond Formation in the Intradiscal Domain. Journal of Molecular Biology. 374 (5), 1309-1318

Kota, P., Reeves, PJ., RajBhandary, UL. and Khorana, HG., (2006). Opsin is present as dimers in COS1 cells: Identification of amino acids at the dimeric interface. Proceedings of the National Academy of Sciences. 103 (9), 3054-3059

Chelikani, P., Reeves, PJ., Rajbhandary, UL. and Khorana, HG., (2006). The synthesis and high-level expression of a β2-adrenergic receptor gene in a tetracycline-inducible stable mammalian cell line. Protein Science. 15 (6), 1433-1440

Zhao, X., Nagai, Y., Reeves, PJ., Kiley, P., Khorana, HG. and Zhang, S., (2006). Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin. Proceedings of the National Academy of Sciences. 103 (47), 17707-17712

Patel, AB., Crocker, E., Reeves, PJ., Getmanova, EV., Eilers, M., Khorana, HG. and Smith, SO., (2005). Changes in Interhelical Hydrogen Bonding upon Rhodopsin Activation. Journal of Molecular Biology. 347 (4), 803-812

Kim, J-M., Hwa, J., Garriga, P., Reeves, PJ., RajBhandary, UL. and Khorana, HG., (2005). Light-Driven Activation of β2-Adrenergic Receptor Signaling by a Chimeric Rhodopsin Containing the β2-Adrenergic Receptor Cytoplasmic Loops†. Biochemistry. 44 (7), 2284-2292

Klein-Seetharaman, J., Yanamala, NVK., Javeed, F., Reeves, PJ., Getmanova, EV., Loewen, MC., Schwalbe, H. and Khorana, HG., (2004). Differential dynamics in the G protein-coupled receptor rhodopsin revealed by solution NMR. Proceedings of the National Academy of Sciences. 101 (10), 3409-3413

Getmanova, E., Patel, AB., Klein-Seetharaman, J., Loewen, MC., Reeves, PJ., Friedman, N., Sheves, M., Smith, SO. and Khorana, HG., (2004). NMR Spectroscopy of Phosphorylated Wild-Type Rhodopsin:  Mobility of the Phosphorylated C-Terminus of Rhodopsin in the Dark and upon Light Activation†. Biochemistry. 43 (4), 1126-1133

Crocker, E., Patel, AB., Eilers, M., Jayaraman, S., Getmanova, E., Reeves, PJ., Ziliox, M., Khorana, HG., Sheves, M. and Smith, SO., (2004). Dipolar assisted rotational resonance NMR of tryptophan and tyrosine in rhodopsin. Journal of Biomolecular NMR. 29 (1), 11-20

Reeves, PJ., Callewaert, N., Contreras, R. and Khorana, HG., (2002). Structure and function in rhodopsin: High-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proceedings of the National Academy of Sciences. 99 (21), 13419-13424

Khorana, HG., Reeves, P. and Kim, J-M., (2002). Structure and Mechanism in G Protein-coupled Receptors. Pharmaceutical News. 9 (5), 287-294

Eilers, M., Ying, W., Reeves, PJ., Gobind Khorana, H. and Smith, SO., (2002). Magic angle spinning nuclear magnetic resonance of isotopically labeled rhodopsin. Methods in Enzymology. 343, 212-222

Reeves, PJ., Kim, J-M. and Khorana, HG., (2002). Structure and function in rhodopsin: A tetracycline-inducible system in stable mammalian cell lines for high-level expression of opsin mutants. Proceedings of the National Academy of Sciences. 99 (21), 13413-13418

Klein-Seetharaman, J., Reeves, PJ., Loewen, MC., Getmanova, EV., Chung, J., Schwalbe, H., Wright, PE. and Khorana, HG., (2002). Solution NMR spectroscopy of [α-15N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence. Proceedings of the National Academy of Sciences. 99 (6), 3452-3457

Loewen, MC., Klein-Seetharaman, J., Getmanova, EV., Reeves, PJ., Schwalbe, H. and Khorana, HG., (2001). Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin. Proceedings of the National Academy of Sciences. 98 (9), 4888-4892

Cha, K., Reeves, PJ. and Khorana, HG., (2000). Structure and function in rhodopsin: Destabilization of rhodopsin by the binding of an antibody at the N-terminal segment provides support for involvement of the latter in an intradiscal tertiary structure. Proceedings of the National Academy of Sciences. 97 (7), 3016-3021

Bruel, C., Cha, K., Niu, L., Reeves, PJ. and Khorana, HG., (2000). Rhodopsin kinase: Two mAbs binding near the carboxyl terminus cause time-dependent inactivation. Proceedings of the National Academy of Sciences. 97 (7), 3010-3015

Bruel, C., Cha, K., Reeves, PJ., Getmanova, E. and Khorana, HG., (2000). Rhodopsin kinase: Expression in mammalian cells and a two-step purification. Proceedings of the National Academy of Sciences. 97 (7), 3004-3009

Hwa, J., Reeves, PJ., Klein-Seetharaman, J., Davidson, F. and Khorana, HG., (1999). Structure and function in rhodopsin: Further elucidation of the role of the intradiscal cysteines, Cys-110, -185, and -187, in rhodopsin folding and function. Proceedings of the National Academy of Sciences. 96 (5), 1932-1935

Reeves, PJ., Hwa, J. and Khorana, HG., (1999). Structure and function in rhodopsin: Kinetic studies of retinal binding to purified opsin mutants in defined phospholipid-detergent mixtures serve as probes of the retinal binding pocket. Proceedings of the National Academy of Sciences. 96 (5), 1927-1931

Eilers, M., Reeves, PJ., Ying, W., Khorana, HG. and Smith, SO., (1999). Magic angle spinning NMR of the protonated retinylidene Schiff base nitrogen in rhodopsin: Expression of 15N-lysine- and 13C-glycine-labeled opsin in a stable cell line. Proceedings of the National Academy of Sciences. 96 (2), 487-492

Klein-Seetharaman, J., Getmanova, EV., Loewen, MC., Reeves, PJ. and Khorana, HG., (1999). NMR spectroscopy in studies of light-induced structural changes in mammalian rhodopsin: Applicability of solution 19F NMR. Proceedings of the National Academy of Sciences. 96 (24), 13744-13749

Housby, JN., Thomas, JD., Wharam, SD., Reeves, PJ. and Salmond, GPC., (1998). Conditional mutations in OutE and OutL block exoenzyme secretion across theErwinia carotovoraouter membrane. FEMS Microbiology Letters. 165 (1), 91-102

Reeves, PJ., Thurmond, RL. and Khorana, HG., (1997). High-level production of rhodopsin and selected mutants using stable mammalian cell lines. FASEB Journal. 11 (9)

Thomas, JD., Reeves, PJ. and Salmond, GPC., (1997). The general secretion pathway of Erwinia carotovora subsp. carotovora: analysis of the membrane topology of OutC and OutF. Microbiology. 143 (3), 713-720

Reeves, PJ., Thurmond, RL. and Khorana, HG., (1996). Structure and function in rhodopsin: high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines.. Proceedings of the National Academy of Sciences. 93 (21), 11487-11492

Reeves, PJ., Douglas, P. and Salmond, GPC., (1994). Beta-lactamase topology probe analysis of the OutO NMePhe peptidase, and six other Out protein components of the Erwinia carotovora general secretion pathway apparatus. Molecular Microbiology. 12 (3), 445-457

Walker, DS., (1994). The Major Secreted Cellulase, CelV,ofErwinia carotovorasubsp,carotovoraIs an Important Soft Rot Virulence Factor. Molecular Plant-Microbe Interactions. 7 (3), 425-425

Salmond, GPC. and Reeves, PJ., (1993). Membrance traffic wardens and protein secretion in Gram-negative bacteria. Trends in Biochemical Sciences. 18 (1), 7-12

Reeves, PJ., Whitcombe, D., Wharam, S., Gibson, M., Allison, G., Bunce, N., Barallon, R., Douglas, P., Mulholland, V., Stevens, S., Walker, D. and Salmond, GPC., (1993). Molecular cloning and characterization of 13 out genes from Erwinia carotovora subspecies carotovora: genes encoding members of a general secretion pathway (GSP) widespread in Gram-negative bacteria. Molecular Microbiology. 8 (3), 443-456

Mulholland, V., Hinton, JCD., Sidebotham, J., Toth, IK., Hyman, LJ., Perombelon, MCM., Reeves, PJ. and Salmond, GPC., (1993). A pleiotropic reduced virulence (Rvi-) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens. Molecular Microbiology. 10 (5), 1154-1154

Mulholland, V., Hinton, JCD., Sidebotham, J., Toth, IK., Hyman, LJ., Perombelon, MCM., Reeves, PJ. and Salmond, GPC., (1993). A pleiotropic reduced virulence (Rvi?) mutant of Erwinia carotovora subspecies atroseptica is defective in flagella assembly proteins that are conserved in plant and animal bacterial pathogens. Molecular Microbiology. 9 (2), 343-356

Jones, S., Yu, B., Bainton, NJ., Birdsall, M., Bycroft, BW., Chhabra, SR., Cox, AJ., Golby, P., Reeves, PJ. and Stephens, S., (1993). The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa.. The EMBO Journal. 12 (6), 2477-2482

Swift, S., Winson, MK., Chan, PF., Bainton, NJ., Birdsall, M., Reeves, PJ., Rees, CED., Chhabra, SR., Hill, PJ., Throup, JP., Bycroft, BW., Salmond, GPC., Williams, P. and Stewart, GSAB., (1993). A novel strategy for the isolation of luxl homologues: evidence for the widespread distribution of a LuxR:Luxl superfamily in enteric bacteria. Molecular Microbiology. 10 (3), 511-520

Salmond, G. and Reeves, P., (1993). Response from Salmond and Reeves. Trends in Microbiology. 1 (7), 250-251

Book chapters (2)

Kimata, N., Pope, A., Rashid, D., Reeves, PJ. and Smith, SO., (2015). Sequential Structural Changes in Rhodopsin Occurring upon Photoactivation. In: Methods in Molecular Biology. Springer New York. 159- 171. 9781493923298

Goncalves, J., Eilers, M., South, K., Opefi, CA., Laissue, PP., Reeves, PJ. and Smith, SO., (2013). Magic angle spinning nuclear magnetic resonance spectroscopy of G protein-coupled receptors. In: G Protein Coupled Receptors Modeling, Activation, Interactions and Virtual Screening. Editors: Conn, PM., . Elsevier. 365- 389. 9780124078659

Conferences (8)

Athanasiou, D., Pang, G., Bellingham, J., Aguila, M., Opefi, CA., Reeves, PJ. and Cheetham, ME., (2018). Pharmacological stimulation of the cell stress responses as a therapy for rhodopsin RP

Athanasiou, D., Aguila, M., South, K., Bevilacqua, D., Mackenzie, F., Munro, PM., Sherman, MY., Reeves, PJ. and Cheetham, ME., (2015). Improvement of P23H rod opsin traffic after metformin treatment accelerates the rate of retinal degeneration in P23H retinitis pigmentosa

Vickers, AJ., Ma, Y., Dudley, R., Reeves, PJ., Reynolds, CA. and Gadde, S., (2008). THz differential spectroscopy of Rhodopsin

Vickers, AJ., Dudley, R., Reeves, PJ., Reynolds, CA., Gadde, S., Nithyanand, TN. and Ma, Y., (2007). THz time-domain (THz-TDs) spectroscopy of bovine rhodopsin from rod outer segments

Vohra, S., Chintapalli, SV., Illingworth, CJR., Reeves, PJ., Mullineaux, PM., Clark, HSX., Dean, MK., Upton, GJG. and Reynolds, CA., (2007). Computational studies of Family A and Family B GPCRs

Vickers, AJ., Dudley, R., Reeves, PJ. and Ma, Y., (2006). Time-domain THz spectroscopy (TDS-THz) of bovine rhodopsin in solution

Reeves, PJ., Klein-Seetharaman, J., Getmanova, EV., Eilers, M., Loewen, MC., Smith, SO. and Khorana, HG., (1999). Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies

Thurmond, RL., Creuzenet, C., Reeves, PJ. and Khorana, HG., (1997). Structure and function in rhodopsin: Peptide sequences in the cytoplasmic loops of rhodopsin are intimately involved in interaction with rhodopsin kinase

Grants and funding

2019

Mechanisms of GPCR Signaling

National Institutes of Health

2017

Biomedical Vacation Scholarships

Wellcome Trust

Measuring stress in the Endoplasmic Reticulum caused by rhodopsin retinitis pigmentosa mutants

Wellcome Trust

2015

Construction and Spectral Charaterisation of Rhodopsin Autosomal Dominant Retinitis Pigmentosa

Wellcome Trust

2014

Elucidation of Molecular Mechanism(s) of Retinitis Pigmentosa Resulting from Mutations in Rhodopson

Fight for Sight

Biomedical Vacation Scholarships - 2014 (Miss Stan)

Wellcome Trust

2012

Temperature-sensitive misfolding in Rhodopson Retinitis Pigmentosa mutants

Wellcome Trust

2011

Structural restoration of Rhodopsin ADRP mutants

BRPS

Vacation Scholarship: Modupe O Bakare

Wellcome Trust

Undergraduate Research Bursary - Jack Newman

Nuffield Foundation

Structure and Activation Mechanism of the Visual Pigment Rhodopsin

National Institute Of Health (Nih)

2010

Vacation Scholarship: Mang Ching Lai

Wellcome Trust

2009

Solid-State NMR Studies of Membrane Proteins & Coenzymes

The Research Foundation

1900

Mechanisms of GPCR Signaling

National Institutes of Health

Contact

preeves@essex.ac.uk
+44 (0) 1206 873763

Location:

3SW.5.31, Colchester Campus