Clearing 2021
People

Dr Marcus Edwards

Lecturer
School of Life Sciences
Dr Marcus Edwards

Profile

Qualifications

  • PhD John Innes Centre, (2009)

  • MSc - Bioinformatics University of East Anglia, (2005)

  • BSc - Biochemistry University of East Anglia, (2004)

Appointments

University of Essex

  • Lecturer, School of Life Sciences, University of Essex (1/7/2020 - present)

Other academic

  • Researcher Co-Investigator / Senior Research Associate, University of East Anglia (1/10/2009 - 30/6/2020)

Research and professional activities

Research interests

Bacterial Extracellular Electron Transfer

My lab’s research mainly focusses upon bacterial electron transfer. Many bacteria are able to exchange electrons with their extracellular environment. These include iron reducing bacteria, such as Shewanella and Geobacter, which transfer intracellular electrons to iron oxides in order to support anaerobic respiration. These also include iron oxidising bacteria, such as Sideroxydans and Rhodopseudomonas which are able to obtain electrons from reduced iron species outside of the cell in order to generate reducing equivalents (NADH) and chemical energy (ATP) within the cell. The ability of bacteria to directly exchange electrons with their environment not only impacts on the biogeochemical cycling of many redox active elements, but is also of great interest for the potential biotechnological applications including microbial fuel cells, bioelectrosynthesis, biosensors, bioremediation and bioreclamation of metals from waste streams.

Key words: Metalloproteins

Teaching and supervision

Current teaching responsibilities

  • General and Organic Chemistry (BS132)

  • Inorganic and Physical Chemistry (BS133)

  • Employability Skills for the Biosciences (BS211)

  • Protein Bioinformatics (BS281)

  • Research Project in Life Sciences (BS832)

  • Research Project: MSc Biotechnology (BS984)

  • Research Project: MSc Molecular Medicine (BS986)

  • Research Project: MSc Cancer Biology (BS987)

Publications

Journal articles (24)

Van Wonderen, J., Adamczyk, K., Wu, X., Jiang, X., Piper, S., Hall, C., Edwards, M., Clarke, T., Zhang, H., Jeuken, L., Sazanovich, I., Towrie, M., Blumberger, J., Meech, S. and Butt, J., Nanosecond Heme-to-Heme Electron Transfer Rates in a Multiheme Cytochrome Nanowire Reported by a Spectrally Unique His/Met Ligated Heme. Proceedings of the National Academy of Sciences of USA

Piper, SEH., Edwards, MJ., van Wonderen, JH., Casadevall, C., Martel, A., Jeuken, LJC., Reisner, E., Clarke, TA. and Butt, JN., Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit. Frontiers in Microbiology. 12

Clarke, TA. and Edwards, MJ., (2020). Uncovering nature’s electronics. Nature Chemical Biology. 16 (10), 1041-1042

Li, D-B., Edwards, MJ., Blake, AW., Newton-Payne, SE., Piper, SEH., Jenner, LP., Sokol, KP., Reisner, E., Van Wonderen, JH., Clarke, TA. and Butt, JN., (2020). His/Met heme ligation in the PioA outer membrane cytochrome enabling light-driven extracellular electron transfer by Rhodopseudomonas palustris TIE-1. Nanotechnology. 31 (35), 354002-354002

Edwards, MJ., Richardson, DJ., Paquete, CM. and Clarke, TA., (2020). Role of multiheme cytochromes involved in extracellular anaerobic respiration in bacteria. Protein Science. 29 (4), 830-842

Edwards, MJ., White, GF., Butt, JN., Richardson, DJ. and Clarke, TA., (2020). The Crystal Structure of a Biological Insulated Transmembrane Molecular Wire. Cell. 181 (3), 665-673.e10

Jiang, X., van Wonderen, JH., Butt, JN., Edwards, MJ., Clarke, TA. and Blumberger, J., (2020). Which Multi-Heme Protein Complex Transfers Electrons More Efficiently? Comparing MtrCAB from Shewanella with OmcS from Geobacter. Journal of Physical Chemistry Letters. 11 (21), 9421-9425

Edwards, MJ., White, GF., Lockwood, CW., Lawes, MC., Martel, A., Harris, G., Scott, DJ., Richardson, DJ., Butt, JN. and Clarke, TA., (2018). Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners. Journal of Biological Chemistry. 293 (21), 8103-8112

Edwards, MJ., Gates, AJ., Butt, JN., Richardson, DJ. and Clarke, TA., (2017). Comparative structure-potentio-spectroscopy of the Shewanella outer membrane multiheme cytochromes. Current Opinion in Electrochemistry. 4 (1), 199-205

Edwards, MJ., White, GF., Norman, M., Tome-Fernandez, A., Ainsworth, E., Shi, L., Fredrickson, JK., Zachara, JM., Butt, JN., Richardson, DJ. and others, (2015). Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer.. Scientific reports. 5 (1), 11677-11677

Beckwith, CR., Edwards, MJ., Lawes, M., Shi, L., Butt, JN., Richardson, DJ. and Clarke, TA., (2015). Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth. Frontiers in microbiology. 6, 332-332

Liu, Y., Wang, Z., Liu, J., Levar, C., Edwards, MJ., Babauta, JT., Kennedy, DW., Shi, Z., Beyenal, H., Bond, DR. and others, (2014). A trans-outer membrane porin-cytochrome protein complex for extracellular electron transfer by G eobacter sulfurreducens PCA. Environmental microbiology reports. 6 (6), 776-785

Edwards, MJ., Baiden, NA., Johs, A., Tomanicek, SJ., Liang, L., Shi, L., Fredrickson, JK., Zachara, JM., Gates, AJ., Butt, JN. and others, (2014). The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe–mineral interface. FEBS letters. 588 (10), 1886-1890

Hearnshaw, SJ., Edwards, MJ., Stevenson, CE., Lawson, DM. and Maxwell, A., (2014). A new crystal structure of the bifunctional antibiotic simocyclinone D8 bound to DNA gyrase gives fresh insight into the mechanism of inhibition. Journal of molecular biology. 426 (10), 2023-2033

Richardson, DJ., Butt, JN., Fredrickson, JK., Zachara, JM., Shi, L., Edwards, MJ., White, G., Baiden, N., Gates, AJ., Marritt, SJ. and others, (2012). The �porin–cytochrome�model for microbe-to-mineral electron transfer. Molecular microbiology. 85, 201-212

Liu, J., Wang, Z., Belchik, SM., Edwards, MJ., Liu, C., Kennedy, DW., Merkley, ED., Lipton, MS., Butt, JN., Richardson, DJ. and others, (2012). Identification and characterization of MtoA: a decaheme c-type cytochrome of the neutrophilic Fe (II)-oxidizing bacterium Sideroxydans lithotrophicus ES-1. Frontiers in microbiology. 3, 37-37

Edwards, MJ., Hall, A., Shi, L., Fredrickson, JK., Zachara, JM., Butt, JN., Richardson, DJ. and Clarke, TA., (2012). The crystal structure of the extracellular 11-heme cytochrome UndA reveals a conserved 10-heme motif and defined binding site for soluble iron chelates. Structure. 20 (7), 1275-1284

Edwards, MJ., Fredrickson, JK., Zachara, JM., Richardson, DJ. and Clarke, TA., (2012). Analysis of structural MtrC models based on homology with the crystal structure of MtrF. Biochemical Society Transactions. 40 (6), 1181-1185

Breuer, M., Zarzycki, P., Shi, L., Clarke, TA., Edwards, MJ., Butt, JN., Richardson, DJ., Fredrickson, JK., Zachara, JM., Blumberger, J. and others, (2012). Molecular structure and free energy landscape for electron transport in the decahaem cytochrome MtrF. Biochemical Society Transactions. 40 (6), 1198-1203

Richardson, DJ., Butt, JN., Fredrickson, JK., Zachara, JM., Shi, L., Edwards, MJ., White, G., Baiden, N., Gates, AJ., Marritt, SJ. and Clarke, TA., (2012). The ‘porin-cytochrome’ model for microbe-to-mineral electron transfer. Molecular Microbiology. 85 (2), 201-212

Clarke, TA., Edwards, MJ., Gates, AJ., Hall, A., White, GF., Bradley, J., Reardon, CL., Shi, L., Beliaev, AS., Marshall, MJ. and others, (2011). Structure of a bacterial cell surface decaheme electron conduit. Proceedings of the National Academy of Sciences. 108 (23), 9384-9389

Edwards, MJ., Williams, MA., Maxwell, A. and McKay, AR., (2011). Mass spectrometry reveals that the antibiotic simocyclinone D8 binds to DNA gyrase in a �bent-over� conformation: evidence of positive cooperativity in binding. Biochemistry. 50 (17), 3432-3440

Edwards, MJ., Flatman, RH., Mitchenall, LA., Stevenson, CEM., Le, TBK., Clarke, TA., McKay, AR., Fiedler, H-P., Buttner, MJ., Lawson, DM. and others, (2009). A crystal structure of the bifunctional antibiotic simocyclinone D8, bound to DNA gyrase. Science. 326 (5958), 1415-1418

Edwards, MJ., Flatman, RH., Mitchenall, LA., Stevenson, CEM., Maxwell, A. and Lawson, DM., (2009). Crystallization and preliminary X-ray analysis of a complex formed between the antibiotic simocyclinone D8 and the DNA breakage–reunion domain of Escherichia coli DNA gyrase. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 65 (8), 846-848

Book chapters (2)

Lockwood, CWJ., van Wonderen, JH., Edwards, MJ., Piper, SEH., White, GF., Newton-Payne, S., Richardson, DJ., Clarke, TA. and Butt, JN., (2018). Membrane-spanning electron transfer proteins from electrogenic bacteria: Production and investigation. In: Methods in Enzymology. Academic Press. 257- 275

White, GF., Edwards, MJ., Gomez-Perez, L., Richardson, DJ., Butt, JN. and Clarke, TA., (2016). Mechanisms of bacterial extracellular electron exchange. In: Advances in microbial physiology. Academic Press. 87- 138. 978-0-12-804823-8

Other (1)

Richardson, DJ., Edwards, MJ., White, GF., Baiden, N., Hartshorne, RS., Fredrickson, J., Shi, L., Zachara, J., Gates, AJ., Butt, JN. and others, (2012).Exploring the biochemistry at the extracellular redox frontier of bacterial mineral Fe (III) respiration,Portland Press Ltd.

Contact

m.edwards@essex.ac.uk

Location:

Colchester Campus