People

Dr Vassiliy Bavro

Reader
School of Life Sciences
Dr Vassiliy Bavro
  • Email

  • Telephone

    +44 (0) 1206 872243

  • Location

    3SW.5.19, Colchester Campus

  • Academic support hours

    Director MSc Molecular Medicine - BS 5.19 - Tuesday 14:00 -15:00

Profile

Biography

Structural biologist with interests in membrane protein crystallography and integrative structural biology approaches, including structural mass-spectrometry. Research areas cover bacterial transporters involved in antibiotic resistance and ion channels. Protein structure-function relationships and involvement in disease.

Qualifications

  • PhD (supervisor Prof. Winfried Weissenhorn) European Molecular Biology Laboratory, (2003)

  • MSc Biochemistry and Microbiology (supervisor Prof.Shawn Doonan) University of East London, (1996)

  • MSc Biochemistry and Microbiology Sofia University, (1996)

  • BSc Molecular Microbiology Sofia University, (1994)

Appointments

University of Essex

  • Reader in Membrane Protein Science, Biological Sciences, University of Essex (1/5/2016 - present)

  • Convenor Protein Structure and Function Group (PSFG), Biological Sciences, University of Essex (1/8/2017 - present)

  • Director MSc Molecular Medicine, Biological Sciences, University of Essex (1/8/2017 - present)

Other academic

  • Reader in Membrane Protein Science, Biological Sciences, University of Essex (1/5/2016 - present)

  • Director MSc Molecular Medicine, Biological Sciences, University of Essex (1/8/2017 - present)

  • Senior Visiting Research Fellow, School of Medicine, University of Birmingham (1/5/2016 - present)

  • Birmingham Fellow, School of Medicine, University of Birmingham (1/2/2012 - 30/4/2016)

  • PI in Structural Microbiology, School of Medicine, University of Birmingham (2/2012 - 4/2016)

  • Senior Post Doctoral Research Associate, Physics, University of Oxford (2008 - 2012)

  • Marie Curie Post Doctoral Research Fellow, Biochemistry, University of Cambridge (2005 - 2007)

  • Post-Doctoral Research Associate, Biochemistry, University of Cambridge (12/10/2003 - 2005)

Research and professional activities

Research interests

Membrane protein crystallography

Key words: membrane proteins
Open to supervise

Antimicrobial Resistance

Our group is interested in the molecular mechanisms of multidrug resistance provided by the tripartite efflux pumps in Gram-negative bacteria. We are seeking to understand the mechanistic aspects of the assembly and substrate recognition in these multicomponent molecular machines.

Key words: multidrug efflux pumps
Open to supervise

Integrative structural biology approaches

Key words: X-ray radiolytic footprinting
Open to supervise

Ion channels

Key words: Ion channels
Open to supervise

Metal transport in bacteria

Key words: Copper homeostasis
Open to supervise

Conferences and presentations

Gordon Research Conference (GRC) Efflux pumps 2019

Conference, Gordon Research Conference (GRC) Efflux pumps 2019, Lucca, Italy, 29/4/2019

Biophysical Society Conference

Conference, BPS 62 Annual Meeting, San Francisco, United States, 18/2/2018

Immunological assessment of the nature of binding of protective antibodies to the Gram-negative envelope reveals a complex role of LPS O-chain in restricting protection from vaccination.

Invited presentation, Conference, BacPath 14 - Molecular Biology of Bacterial Pathogens 2017, Hahndorf, Australia, 27/9/2017

Teaching and supervision

Current teaching responsibilities

  • Enterprise and Employability for the Biosciences (BS211)

  • Medical Microbiology (BS235)

  • Rational Drug Design (BS317)

  • Biomembranes and Bioenergetics (BS332)

  • Research Project in Biomolecular Science (BS831)

  • Research Project in Ecological and Marine Sciences (BS832)

  • Research Project: MSc Biotechnology (BS984)

  • Professional Skills and the Business of Biosciences (BS985)

  • Research Project: MSc Molecular Medicine (BS986)

Publications

Journal articles (32)

Straw, ML., Chaplin, AK., Hough, MA., Paps, J., Bavro, VN., Wilson, MT., Vijgenboom, E. and Worrall, JAR., (2018). A cytosolic copper storage protein provides a second level of copper tolerance in Streptomyces lividans.. Metallomics: integrated biometal science. 2018 (10), 180-193

Lu, W-J., Lin, H-J., Janganan, TK., Li, C-Y., Chin, W-C., Bavro, VN. and Lin, H-TV., (2018). ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function.. International Journal of Molecular Sciences. 19 (4), 1000-1000

Abdullah, N., Kelly, JT., Graham, SC., Birch, J., Gonçalves-Carneiro, D., Mitchell, T., Thompson, RN., Lythgoe, KA., Logan, N., Hosie, MJ., Bavro, VN., Willett, BJ., Heaton, MP. and Bailey, D., (2018). Structure-Guided Identification of a Nonhuman Morbillivirus with Zoonotic Potential. Journal of Virology. 92 (23)

Sen, H., Aggarwal, N., Ishionwu, C., Hussain, N., Parmar, C., Jamshad, M., Bavro, VN. and Lund, PA., (2017). Structural and functional analysis of the Escherichia coli acid-sensing histidine kinase EvgS. Journal of Bacteriology. 199 (18)

Bernardes, A., Textor, LC., Santos, JC., Cuadrado, NH., Kostetsky, EY., Roig, MG., Bavro, VN., Muniz, JRC., Shnyrov, VL. and Polikarpov, I., (2015). Crystal structure analysis of peroxidase from the palm tree Chamaerops excelsa. Biochimie. 111, 58-69

Zubcevic, L., Wang, S., Bavro, VN., Lee, SJ., Nichols, CG. and Tucker, SJ., (2015). Modular design of the selectivity filter pore loop in a novel family of prokaryotic inward rectifier' (NirBac) channels. Scientific Reports. 5 (1), 15305-

Symmons, MF., Marshall, RL. and Bavro, VN., (2015). Architecture and roles of periplasmic adaptor proteins in tripartite efflux assemblies. Frontiers in Microbiology. 6 (MAY), 513-

Browning, DF., Bavro, VN., Mason, JL., Sevastsyanovich, YR., Rossiter, AE., Jeeves, M., Wells, TJ., Knowles, TJ., Cunningham, AF., Donald, JW., Palmer, T., Overduin, M. and Henderson, IR., (2015). Cross-species chimeras reveal BamA POTRA and β-barrel domains must be fine-tuned for efficient OMP insertion. Molecular Microbiology. 97 (4), 646-659

Bavro, VN., Gupta, S. and Ralston, C., (2015). Oxidative footprinting in the study of structure and function of membrane proteins: Current state and perspectives. Biochemical Society Transactions. 43 (5), 983-994

Blair, JMA., Bavro, VN., Ricci, V., Modi, N., Cacciotto, P., Kleinekathӧfer, U., Ruggerone, P., Vargiu, AV., Baylay, AJ., Smith, HE., Brandon, Y., Galloway, D. and Piddock, LJV., (2015). AcrB drug-binding pocket substitution confers clinically relevant resistance and altered substrate specificity. Proceedings of the National Academy of Sciences. 112 (11), 3511-3516

Zubcevic, L., Bavro, VN., Muniz, JRC., Schmidt, MR., Wang, S., De Zorzi, R., Venien-Bryan, C., Sansom, MSP., Nichols, CG. and Tucker, SJ., (2014). Control of KirBac3.1 Potassium Channel Gating at the Interface between Cytoplasmic Domains. Journal of Biological Chemistry. 289 (1), 143-151

Weeks, JW., Bavro, VN. and Misra, R., (2014). Genetic assessment of the role of AcrB β-hairpins in the assembly of the TolC-AcrAB multidrug efflux pump ofEscherichia coli. Molecular Microbiology. 91 (5), 965-975

Janganan, TK., Bavro, VN., Zhang, L., Borges-Walmsley, MI. and Walmsley, AR., (2013). Tripartite efflux pumps: energy is required for dissociation, but not assembly or opening of the outer membrane channel of the pump. Molecular Microbiology. 88 (3), 590-602

Browning, DF., Matthews, SA., Rossiter, AE., Sevastsyanovich, YR., Jeeves, M., Mason, JL., Wells, TJ., Wardius, CA., Knowles, TJ., Cunningham, AF., Bavro, VN., Overduin, M. and Henderson, IR., (2013). Mutational and Topological Analysis of the Escherichia coli BamA Protein. PLoS ONE. 8 (12), e84512-e84512

Bavro, VN., De Zorzi, R., Schmidt, MR., Muniz, JRC., Zubcevic, L., Sansom, MSP., Vénien-Bryan, C. and Tucker, SJ., (2012). Structure of a KirBac potassium channel with an open bundle crossing indicates a mechanism of channel gating. Nature Structural & Molecular Biology. 19 (2), 158-163

Janganan, TK., Zhang, L., Bavro, VN., Matak-Vinkovic, D., Barrera, NP., Burton, MF., Steel, PG., Robinson, CV., Borges-Walmsley, MI. and Walmsley, AR., (2011). Opening of the Outer Membrane Protein Channel in Tripartite Efflux Pumps Is Induced by Interaction with the Membrane Fusion Partner. Journal of Biological Chemistry. 286 (7), 5484-5493

Janganan, TK., Bavro, VN., Zhang, L., Matak-Vinkovic, D., Barrera, NP., Venien-Bryan, C., Robinson, CV., Borges-Walmsley, MI. and Walmsley, AR., (2011). Evidence for the Assembly of a Bacterial Tripartite Multidrug Pump with a Stoichiometry of 3:6:3. Journal of Biological Chemistry. 286 (30), 26900-26912

Gupta, S., Bavro, VN., D'Mello, R., Tucker, SJ., Vénien-Bryan, C. and Chance, MR., (2010). Conformational Changes During the Gating of a Potassium Channel Revealed by Structural Mass Spectrometry. Structure. 18 (7), 839-846

Wang, SC., Politis, A., Di Bartolo, N., Bavro, VN., Tucker, SJ., Booth, PJ., Barrera, NP. and Robinson, CV., (2010). Ion Mobility Mass Spectrometry of Two Tetrameric Membrane Protein Complexes Reveals Compact Structures and Differences in Stability and Packing. Journal of the American Chemical Society. 132 (44), 15468-15470

Paynter, JJ., Andres-Enguix, I., Fowler, PW., Tottey, S., Cheng, W., Enkvetchakul, D., Bavro, VN., Kusakabe, Y., Sansom, MSP., Robinson, NJ., Nichols, CG. and Tucker, SJ., (2010). Functional Complementation and Genetic Deletion Studies of KirBac Channels. Journal of Biological Chemistry. 285 (52), 40754-40761

Barrera, NP., Isaacson, SC., Zhou, M., Bavro, VN., Welch, A., Schaedler, TA., Seeger, MA., Miguel, RN., Korkhov, VM., van Veen, HW., Venter, H., Walmsley, AR., Tate, CG. and Robinson, CV., (2009). Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions. Nature Methods. 6 (8), 585-587

Misra, R. and Bavro, VN., (2009). Assembly and transport mechanism of tripartite drug efflux systems. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1794 (5), 817-825

Lin, HT., Bavro, VN., Barrera, NP., Frankish, HM., Velamakanni, S., van Veen, HW., Robinson, CV., Borges-Walmsley, MI. and Walmsley, AR., (2009). MacB ABC Transporter Is a Dimer Whose ATPase Activity and Macrolide-binding Capacity Are Regulated by the Membrane Fusion Protein MacA. Journal of Biological Chemistry. 284 (2), 1145-1154

Lin, HT., Bavro, VN., Barrera, NP., Frankish, HM., Velamakanni, S., van Veen, HW., Robinson, CV., Borges-Walmsley, MI. and Walmsley, AR., (2009). Erratum: MacB ABC transporter is a dimer whose ATPase activitity and macrolide-binding capacity are regulated by the membrane fusion protein MacA (Journal of Biological Chemistry (2009) 284 (1145-1154)). Journal of Biological Chemistry. 284 (13), 8995-

Lin, HT., Bavro, VN., Barrera, NP., Frankish, HM., Velamakanni, S., van Veen, HW., Robinson, CV., Borges-Walmsley, MI. and Walmsley, AR., (2009). MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA (vol 284, pg 1145, 2009). JOURNAL OF BIOLOGICAL CHEMISTRY. 284 (13), 8995-8995

Pietras, Z., Bavro, V., Furnham, N., Pellegrini-Calace, M., Milner-White, E. and Luisi, B., (2008). Structure and Mechanism of Drug Efflux Machinery in Gram Negative Bacteria. Current Drug Targets. 9 (9), 719-728

Worrall, JAR., Górna, M., Crump, NT., Phillips, LG., Tuck, AC., Price, AJ., Bavro, VN. and Luisi, BF., (2008). Reconstitution and Analysis of the Multienzyme Escherichia coli RNA Degradosome. Journal of Molecular Biology. 382 (4), 870-883

Bavro, VN., Pietras, Z., Furnham, N., Pérez-Cano, L., Fernández-Recio, J., Pei, XY., Misra, R. and Luisi, B., (2008). Assembly and Channel Opening in a Bacterial Drug Efflux Machine. Molecular Cell. 30 (1), 114-121

Mokrab, Y., Bavro, VN., Mizuguchi, K., Todorov, NP., Martin, IL., Dunn, SMJ., Chan, SL. and Chau, P-L., (2007). Exploring ligand recognition and ion flow in comparative models of the human GABA type A receptor. Journal of Molecular Graphics and Modelling. 26 (4), 760-774

Fernandez-Recio, J., Walas, F., Federici, L., Venkatesh Pratap, J., Bavro, VN., Miguel, RN., Mizuguchi, K. and Luisi, B., (2004). A model of a transmembrane drug-efflux pump from Gram-negative bacteria. FEBS Letters. 578 (1-2), 5-9

Sola, M., Bavro, VN., Timmins, J., Franz, T., Ricard-Blum, S., Schoehn, G., Ruigrok, RWH., Paarmann, I., Saiyed, T., O'Sullivan, GA., Schmitt, B., Betz, H. and Weissenhorn, W., (2004). Structural basis of dynamic glycine receptor clustering by gephyrin. The EMBO Journal. 23 (13), 2510-2519

Bavro, VN., Sola, M., Bracher, A., Kneussel, M., Betz, H. and Weissenhorn, W., (2002). Crystal structure of the GABAA-receptor-associated protein, GABARAP. EMBO reports. 3 (2), 183-189

Book chapters (1)

Zgurskaya, HI., Bavro, VN., Weeks, JW. and Krishnamoorthy, G., (2016). Multidrug Efflux in the Context of Two-Membrane Cell Envelopes. In: Efflux-Mediated Antimicrobial Resistance in Bacteria. Editors: Li, XZ., Elkins, C. and Zgurskaya, H., . Springer. 99- 128. 978-3-319-39656-9

Conferences (3)

Grassi, F., Bavro, VN. and Kleinekathöfer, U., (2016). What Keeps TolC Closed? Insights from Molecular Dynamics Simulations

Bavro, VN., De Zorzi, R., Schmidt, MR., Muniz, JRC., Zubcevic, L., Sansom, MSP., Venien-Bryan, C. and Tucker, SJ., (2012). Crystal Structure of a Prokaryotic Kir Channel in an Open Conformation

Gupta, S., D'Mello, R., Chance, MR., Bavro, VN., Venien-Bryan, C. and Tucker, SJ., (2010). Radiolytic Footprinting Reveals Conformational Changes During Potassium Channel Gating

Grants and funding

2019

Protein Detection Kit

East Suffolk and North Essex NHS Foundation Trust

2016

Mapping the interactions within multidrug efflux pump assemblies

Biotechnology & Biology Science Res.Council

Elucidating the interaction of antibiotics with multidrug efflux-pumps

Wellcome Trust

Contact

v.bavro@essex.ac.uk
+44 (0) 1206 872243

Location:

3SW.5.19, Colchester Campus

Academic support hours:

Director MSc Molecular Medicine - BS 5.19 - Tuesday 14:00 -15:00