People

Dr Filippo Prischi

Lecturer
School of Life Sciences
Dr Filippo Prischi
  • Email

  • Telephone

    +44 (0) 1206 873370

  • Location

    3SW.5.03, Colchester Campus

  • Academic support hours

    Open door policy

Profile

Biography

My main research interest is the characterisation of protein complexes that are part of signalling pathways, in normal and cancer cells. My goal is to understand how the molecular machines that compose signalling pathways work together to transfer information. In my group, we use a cutting-edge multi-disciplinary approach that combines biophysical, enzymatic and structural studies. We use X-ray crystallography, Nuclear Magnetic Resonance and Electron Microscopy to visualise macromolecules at atomic and near atomic resolution. At this scale we are able to dissect cellular processes at a molecular level and describe the details at the base of cancer formation or development, thereby providing a solid base for medical advance. The main focus of our research is the study of phosphorylation cascades and protein kinases.

Qualifications

  • Fellow of the Higher Education Academy University of Essex,

  • Executive Education in Project Management University College London, (2013)

  • PhD University of Siena and National Institute for Medical Research (MRC), (2010)

  • M.Sc. Molecular and Cell Biology (110 summa cum laude) University of Siena, (2005)

  • B.Sc. Biological Sciences (110 summa cum laude) University of Siena, (2003)

Appointments

University of Essex

  • Lecturer, School of Biological Sciences, University of Essex (1/6/2015 - present)

Other academic

  • Research Associate, Imperial College London (1/5/2010 - 30/4/2015)

Research and professional activities

Research interests

Signalling pathways in lung and breast cancer

Key words: Kinase
Open to supervise

Drug Discovery

Key words: X-Ray crystallography
Open to supervise

Protein Phosphoregulation

Key words: kinase
Open to supervise

Structural Biology

Key words: X-Ray
Open to supervise

Ribosomal Kinases pathways

Open to supervise

Current research

Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer

Understanding how RSKs regulate Transcription Factors in Triple Negative Breast Cancer

The role of 90kDa Ribosomal S6 Kinases in Steroid signalling

Flipping the switch; regulating protein synthesis in response to stress

Conferences and presentations

Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer

FEBS Congress 2019, Krakow, Poland, 10/7/2019

Purification of protein kinases for structural studies

Invited presentation, AKTA user day, Cambridge, Cambridge, United Kingdom, 21/9/2018

The black sheep of the p90 ribosomal kinase family: RSK4

Invited presentation, 5th EEMaX Symposium, 5th EEMaX Symposium, Colchester, United Kingdom, 20/12/2016

Interaction of BiP and ER stress transducers is disrupted by unfolded proteins causing UPR activation.

16th International MST Meeting, 16th International MST Meeting, Munich, Germany, 18/6/2015

Phosphoregulation of human Ire1 RNase splicing activity.

CSB Open Day, CSB Open Day, London, United Kingdom, 6/6/2014

Phosphoregulation of human Ire1 RNase splicing activity.

Molecular Chaperones and Stress Responses, Molecular Chaperones and Stress Responses, Cold Spring Harbor, United States, 5/2014

Biochemical and Biophysical characterisation of c-IRE1 alpha

OPPF-MPL HTP Protein Production and Crystallization, Harwell, United Kingdom, 4/2011

Insights into the structure of an IscS/IscU/CyaY complex.

Invited presentation, Frataxin meeting, London, United Kingdom, 5/2010

Understanding the iron-sulphur cluster machinery: Characterization of the E. coli IscS/IscU complex.

IIX CCPN meeting, Penrith, United Kingdom, 8/2008

Key features of EF-HAND superfamily structure using paramagnetic probe.

XXXVII National NMR Congress, Verbania Pallanza, Italy, 9/2007

Dimerization of alpha-bungarotoxin monitored by paramagnetic probes: a new approach for protein-protein interaction studies.

Invited presentation, XXXVI National NMR Congress, Vietri Sul Mare, Italy, 9/2006

Teaching and supervision

Current teaching responsibilities

  • Biochemistry of Macromolecules (BS131)

  • Transferable Skills in Life Sciences (BS143)

  • Enterprise and Employability for the Biosciences (BS211)

  • Protein Bioinformatics (BS281)

  • Research Project in Biomolecular Science (BS831)

Previous supervision

Ryan Cronin
Ryan Cronin
Thesis title: The Role of P90 Ribosomal S6 Kinases (Rsks) in Steroid Receptor Signalling
Degree subject: Biochemistry
Degree type: Master of Science (by Dissertation)
Awarded date: 28/6/2019

Publications

Journal articles (20)

Zabet, NR., Catoni, M., Prischi, F. and Paszkowski, J., (2017). Cytosine methylation at CpCpG sites triggers accumulation of non-CpG methylation in gene bodies. Nucleic Acids Research. 45 (7), gkw1330-gkw1330

Prischi, F. and Pastore, A., (2017). Hybrid Methods in Iron-Sulfur Cluster Biogenesis. Frontiers in Molecular Biosciences. 4 (12), 12-

Prischi, F. and Pastore, A., (2016). Application of Nuclear Magnetic Resonance and Hybrid Methods to Structure Determination of Complex Systems. ADVANCED TECHNOLOGIES FOR PROTEIN COMPLEX PRODUCTION AND CHARACTERIZATION. 896, 351-368

Carrara, M., Prischi, F., Nowak, PR., Kopp, MC. and Ali, MMU., (2015). Noncanonical binding of BiP ATPase domain to Ire1 and Perk is dissociated by unfolded protein CH1 to initiate ER stress signaling. eLife. 4 (4)

Popovic, M., Sanfelice, D., Pastore, C., Prischi, F., Temussi, PA. and Pastore, A., (2015). Selective observation of the disordered import signal of a globular protein by in-cell NMR: The example of frataxins. Protein Science. 24 (6), 996-1003

Carrara, M., Prischi, F., Nowak, PR. and Ali, MMU., (2015). Crystal structures reveal transient PERK luminal domain tetramerization in endoplasmic reticulum stress signaling. The EMBO Journal. 34 (11), 1589-1600

Prischi, F., Nowak, PR., Carrara, M. and Ali, MMU., (2014). Phosphoregulation of Ire1 RNase splicing activity. Nature Communications. 5 (1), 3554-

Carrara, M., Prischi, F. and Ali, M., (2013). UPR Signal Activation by Luminal Sensor Domains. International Journal of Molecular Sciences. 14 (3), 6454-6466

Bernini, A., Spiga, O., Venditti, V., Prischi, F., Botta, M., Croce, G., Tong, AP-L., Wong, W-T. and Niccolai, N., (2012). The use of a ditopic Gd(III) paramagnetic probe for investigating α-bungarotoxin surface accessibility. Journal of Inorganic Biochemistry. 112, 25-31

Prischi, F., Konarev, PV., Iannuzzi, C., Pastore, C., Adinolfi, S., Martin, SR., Svergun, DI. and Pastore, A., (2010). Structural bases for the interaction of frataxin with the central components of iron–sulphur cluster assembly. Nature Communications. 1 (1), 95-

Prischi, F., Pastore, C., Carroni, M., Iannuzzi, C., Adinolfi, S., Temussi, P. and Pastore, A., (2010). Of the vulnerability of orphan complex proteins: The case study of the E. coli IscU and IscS proteins. Protein Expression and Purification. 73 (2), 161-166

Prischi, F., Giannini, C., Adinolfi, S. and Pastore, A., (2009). The N-terminus of mature human frataxin is intrinsically unfolded. FEBS Journal. 276 (22), 6669-6676

Adinolfi, S., Iannuzzi, C., Prischi, F., Pastore, C., Iametti, S., Martin, SR., Bonomi, F. and Pastore, A., (2009). Bacterial frataxin CyaY is the gatekeeper of iron-sulfur cluster formation catalyzed by IscS. Nature Structural & Molecular Biology. 16 (4), 390-396

Bernini, A., Venditti, V., Spiga, O., Ciutti, A., Prischi, F., Consonni, R., Zetta, L., Arosio, I., Fusi, P., Guagliardi, A. and Niccolai, N., (2008). NMR studies on the surface accessibility of the archaeal protein Sso7d by using TEMPOL and Gd(III)(DTPA-BMA) as paramagnetic probes. Biophysical Chemistry. 137 (2-3), 71-75

Venditti, V., Bernini, A., De Simone, A., Spiga, O., Prischi, F. and Niccolai, N., (2007). MD and NMR studies of α-bungarotoxin surface accessibility. Biochemical and Biophysical Research Communications. 356 (1), 114-117

Bernini, A., Spiga, O., Venditti, V., Prischi, F., Bracci, L., Tong, AP-L., Wong, W-T. and Niccolai, N., (2006). NMR Studies of Lysozyme Surface Accessibility by Using Different Paramagnetic Relaxation Probes. Journal of the American Chemical Society. 128 (29), 9290-9291

Spiga, O., Padula, MG., Scarselli, M., Ciutti, A., Bernini, A., Venditti, V., Prischi, F., Falciani, C., Lozzi, L., Bracci, L., Valensin, PE., Caudai, C. and Niccolai, N., (2006). Structurally driven selection of human hepatitis C virus mimotopes. Antiviral Therapy. 11 (7), 917-922

Bernini, A., Spiga, O., Venditti, V., Prischi, F., Bracci, L., Huang, J., Tanner, JA. and Niccolai, N., (2006). Tertiary structure prediction of SARS coronavirus helicase. Biochemical and Biophysical Research Communications. 343 (4), 1101-1104

Bernini, A., Spiga, O., Ciutti, A., Venditti, V., Prischi, F., Governatori, M., Bracci, L., Lelli, B., Pileri, S., Botta, M., Barge, A., Laschi, F. and Niccolai, N., (2006). NMR studies of BPTI aggregation by using paramagnetic relaxation reagents. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1764 (5), 856-862

Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Menciassi, N., Finetti, F., Causarono, V., Anselmi, F., Prischi, F. and Niccolai, N., (2003). Molecular modelling of S1 and S2 subunits of SARS coronavirus spike glycoprotein. Biochemical and Biophysical Research Communications. 310 (1), 78-83

Conferences (2)

Prischi, F., Chrysostomou, S., Roy, R., Chapman, K., Mufti, U., Peach, R., Ding, L., Mauri, F., Bellezza, G., Cagini, L., Barbareschi, M., Ferrero, S., Abrahams, J., Ottaviani, S., Castellano, L., Giamas, G., Pascoe, J., Moonamale, D., Billingham, L., Cullen, M., Hrouda, D., Winkler, M., Klug, D., Yaliraki, S., Barahona, M., Wang, Y., Ali, M., Seckl, M. and Pardo, O., (2019). Targeting RSK4 prevents both chemoresistance and metastasis in lung and bladder cancer

Chrysostomou, S., Roy, R., Prischi, F., Chapman, K., Mufti, U., Mauri, F., Bellezza, G., Abrahams, J., Ottaviani, S., Castellano, L., Giamas, G., Hrouda, D., Winkler, M., Klug, D., Yaliraki, S., Barahona, M., Wang, Y., Ali, M., Seckl, M. and Pardo, O., (2019). Abstract 1775: Targeting RSK4 prevents both chemoresistance and metastasis in lung cancer

Reports and Papers (1)

Bavro, V., Trampari, E., Webber, M., Holden, E., Wickham, G., Anuradha, R., Prischi, F., de Oliveira Martins, L. and Savva, G., (2019). Antibiotics select for novel pathways of resistance in biofilms

Dataset (11)

Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F., Venditti, V. and Niccolai, N., Theoretical model of H4 influenza virus

Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F., Venditti, V. and Niccolai, N., Human Influenza B virus trimer structure

Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F., Venditti, V. and Niccolai, N., H5 influenza trimer structure

SPIGA, O., BERNINI, A., CIUTTI, A., CHIELLINI, S., Menciassi, N., Finetti, F., Causarono, V., Anselmi, F., PRISCHI, F. and NICCOLAI, N., S2 SUBUNIT MODEL OF SARS CORONAVIRUS S PROTEIN

Spiga, O., Bernini, A., Prischi, F., Venditti, V., Huang, JD. and Niccolai, N., Theoretical model of SARS Coronavirus helicase

Spiga, O., Bernini, A., Ciutti, A., Chiellini, S., Prischi, F. and Niccolai, N., S1 subunit model of SARS coronavirus S protein

Carrara, M., Prischi, F. and Ali, MMU., Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling

Carrara, M., Prischi, F. and Ali, MMU., Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling

Prischi, F. and Ali, MM., RSK4 N-terminal Kinase Domain in complex with AMP-PNP

Prischi, F. and Ali, MM., Phosphorylated RSK4 N-terminal Kinase Domain in complex with AMP-PNP

Prischi, F. and Ali, MM., RSK4 N-terminal Kinase Domain S232E in complex with AMP-PNP

Grants and funding

2019

Protein Detection Kit

East Suffolk and North Essex NHS Foundation Trust

Development of novel cancer treatments: structural studies of Nectin 4 in complex with bespoke anti-cancer peptides

Bicycle Therapeutics

2018

Flipping the switch; regulating protein synthesis in response to stress

Leverhulme Trust

2016

Phosphoregulation of Transcription Factors in Triple Negative Breast Cancer

Wellcome Trust

Contact

fprischi@essex.ac.uk
+44 (0) 1206 873370

Location:

3SW.5.03, Colchester Campus

Academic support hours:

Open door policy