Professor Philip Mullineaux

Head of School - Professor (R)
School of Biological Sciences
Professor Philip Mullineaux
  • Email

  • Telephone

    +44 (0) 1206 872118

  • Location

    3SW.4.34, Colchester Campus

  • Academic support hours




1981 PhD University of Wales (Swansea) Thesis title: The relationship between photosynthesis and nitrogen fixation in the cyanobacterium Gloeocapsa (Gloeothece) CCAP sp 1430/3. 1981-1983 MRC Research Fellow, University of Edinburgh 1983-1986 Agrigenetics-Funded SSO at John Innes Institute 1986-1989 Complemented SSO (BBSRC) post John Innes Institute 1989-2000 Complemented post: Project Leader (band 4) John Innes Centre 2000-2004 Associate Head of Dept. of Disease and Stress Biology, John Innes Centre 1st June 2004-present. Professor of Plant Molecular Biology, University of Essex. 1st August 2008 - 31st July 2011 Direct of Research, School of Biological Sciences, University of Essex Member of American Society of Plant Biology, Society for Experimental Biology and Society of Free Radical Research Referee for Nature, Science, PNAS, Plant Cell, Plant Journal, Plant Physiology, Journal of Experimental Botany, Physiologia Plantarum, New Phytologist, Plant Molecular Biology As a consequence of living in a constantly changing environment, from which they cannot move, plants have to have sophisticated means of monitoring and responding to changes in their environment. A major aspect of my research is focused on defining the signalling pathways that initiate these responses. An important consequence of such studies is to understand how signalling translates into altered physiological functions of the plants such that they become acclimated to their new conditions. One particular challenge is to translate what we learn in the laboratory to the response of the plant in the field or in its natural habitat. Particularly pertinent are questions about the functioning of signalling networks when the plant is challenged with multiple and fluctuating changes in the environment. In order to begin to understand this we are beginning to work with experimental systems that challenge the plant sequentially or simultaneously with more than one stress. Analysis of the genes that are altered in their expression and the signalling pathways that regulate them are then compared with plants challenged conventionally with a single stress.The final challenge is to move or research from the laboratory to thefield Our initial studies have concentrated on the response of Arabidopsis thaliana (thale cress) to a sudden increase in light intensity. This is a continual problem that plants face, and as a consequence they often absorb more light energy than they can use for photosynthesis. To get around this problem, plants dissipate excess light energy (known as excitation energy) using a variety of processes. Some of these processes (such as the water-water cycle and photorespiration) generate reactive oxygen species (ROS) which are removed by a network of lower molecular weight antioxidants (eg. glutathione and ascorbate) and enzymes (such as superoxide dismutases and ascorbate peroxidases). If the production of ROS exceeds the capacity of the antioxidant network to deal with them, then cells suffer oxidative damage, which is often manifested as bleaching, paling or bronzing of leaves. Therefore a key part of how plants acclimate to such conditions is to adjust the functioning of their antioxidant network to suit their situation. The signal for making these adjustments is the ROS themselves and separate signalling routes controlled by the thiol antioxidant glutathione. An important part of acclimation to a changed environment may include the setting of thresholds for when such defences are deployed. The Mullineaux laboratory moved from the John Innes Centre to the University of Essex on 1st June 2004 and this has provided additional opportunities to initiate research into the genes underlying and influencing water productivity, i.e. the amount of lifetime water consumed as a function of its harvestable product. This work has led to patent filingon the role of a transcription factor in this response. Funding by BBSRC in the SABR initiative has allows us, in collaboration with the Universities of Exeter and Warwick, to begin to develop a Systems Biology approach leading to aholistic view of how signalling pathways integrate and are coordinated to elicit a response at the whole plant level to high light.


  • BSc (Biochemistry) Class 1 University of Wales 1978

  • PhD (Biochemistry) University of Wales 1981

Research and professional activities

Research interests

Signalling pathways that control defence gene expression in plants subject to high light stress

Redox-mediated coordination of abiotic and biotic stress defence signalling pathways with special reference to heat shock transcription factors

Drought responsive gene expression in legumes and Arabidopsis thaliana (thale cress)

Identification of novel genes and processes from C3 desert plants that can be exploited for biotechnological applications

Systems biology led modelling of high light responses and interaction with the regualtion of basla immunity in Arabidopsis

Development of genetic probes based on GFP for non-invasive detection and quantification of hydrogen peroxide accumulation in subcellular compartments

Teaching and supervision

  • Plant Biotechnology (BS323)


Journals (132)

Mullineaux, PM., Exposito-Rodriguez, M., Laissue, PP. and Smirnoff, N., (2018). ROS-dependent signaling pathways in plants and algae exposed to high light: Comparisons with other eukaryotes. Free Radical Biology and Medicine

Exposito-Rodriguez, M., Laissue, PP., Yvon-Durocher, G., Smirnoff, N. and Mullineaux, PM., (2017). Photosynthesis-dependent H₂O₂ transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nature Communications. 8 (1)

Exposito-Rodriguez, M., Laissue, PP., López-Calcagno, PE., Mullineaux, PM., Raines, CA. and Simkin, AJ., (2017). Development of pGEMINI, a Plant Gateway Destination Vector Allowing the Simultaneous Integration of Two cDNA via a Single LR-Clonase Reaction. Plants. 6 (4)

Bechtold, U., Penfold, CA., Jenkins, DJ., Legaie, R., Moore, JD., Lawson, T., Matthews, JSA., Vialet-Chabrand, SRM., Baxter, L., Subramaniam, S., Hickman, R., Florance, H., Sambles, C., Salmon, DL., Feil, R., Bowden, L., Hill, C., Baker, NR., Lunn, JE., Finkenstädt, B., Mead, A., Buchanan-Wollaston, V., Beynon, J., Rand, DA., Wild, DL., Denby, KJ., Ott, S., Smirnoff, N. and Mullineaux, PM., (2016). Time-series transcriptomics reveals that AGAMOUS-LIKE22 affects primary metabolism and developmental processes in drought-stressed arabidopsis. Plant Cell. 28 (2)

Yates, SA., Chernukhin, I., Alvarez-Fernandez, R., Bechtold, U., Baeshen, M., Baeshen, N., Mutwakil, MZ., Sabir, J., Lawson, T. and Mullineaux, PM., (2014). The temporal foliar transcriptome of the perennial C3 desert plant Rhazya stricta in its natural environment. BMC Plant Biology. 14 (1)

Lawson, T., Davey, PA., Yates, SA., Bechtold, U., Baeshen, M., Baeshen, N., Mutwakil, MZ., Sabir, J., Baker, NR. and Mullineaux, PM., (2014). C₃ photosynthesis in the desert plant Rhazya stricta is fully functional at high temperatures and light intensities. New Phytologist. 201 (3)

Gorecka, M., Alvarez-Fernandez, R., Slattery, K., McAusland, L., Davey, PA., Karpinski, S., Lawson, T. and Mullineaux, PM., (2014). Abscisic acid signalling determines susceptibility of bundle sheath cells to photoinhibition in high light-exposed Arabidopsis leaves. Philosophical Transactions of the Royal Society B: Biological Sciences. 369 (1640)

Ryan, AC., Hewitt, CN., Possell, M., Vickers, CE., Purnell, A., Mullineaux, PM., Davies, WJ. and Dodd, IC., (2014). Isoprene emission protects photosynthesis but reduces plant productivity during drought in transgenic tobacco ( Nicotiana tabacum ) plants. New Phytologist. 201 (1)

Bechtold, U., Albihlal, WS., Lawson, T., Fryer, MJ., Sparrow, PA., Richard, F., Persad, R., Bowden, L., Hickman, R., Martin, C., Beynon, JL., Buchanan-Wollaston, V., Baker, NR., Morison, JI., Schöffl, F., Ott, S. and Mullineaux, PM., (2013). Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection. Journal of Experimental Botany. 64 (11)

Bermudez, MA., Galmes, J., Moreno, I., Mullineaux, PM., Gotor, C. and Romero, LC., (2012). Photosynthetic Adaptation to Length of Day Is Dependent on S-Sulfocysteine Synthase Activity in the Thylakoid Lumen. PLANT PHYSIOLOGY. 160 (1)

VICKERS, CE., POSSELL, M., LAOTHAWORNKITKUL, J., RYAN, AC., HEWITT, CN. and MULLINEAUX, PM., (2011). Isoprene synthesis in plants: lessons from a transgenic tobacco model. Plant, Cell & Environment. 34 (6)

Miozzi, L., Catoni, M., Fiorilli, V., Mullineaux, PM., Accotto, GP. and Lanfranco, L., (2011). Arbuscular Mycorrhizal Symbiosis Limits Foliar Transcriptional Responses to Viral Infection and Favors Long-Term Virus Accumulation. Molecular Plant-Microbe Interactions. 24 (12)

Carvalho, LC., Vilela, BJ., Mullineaux, PM. and Amâncio, S., (2011). Comparative Transcriptomic Profiling of Vitis vinifera Under High Light Using a Custom-Made Array and the Affymetrix GeneChip. Molecular Plant. 4 (6)

Mullineaux, PM. and Baker, NR., (2010). Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?. PLANT PHYSIOLOGY. 154 (2)

Galvez-Valdivieso, G. and Mullineaux, PM., (2010). The role of reactive oxygen species in signalling from chloroplasts to the nucleus. Physiologia Plantarum. 138 (4)

Vickers, CE., Possell, M., Nicholas Hewitt, C. and Mullineaux, PM., (2010). Genetic structure and regulation of isoprene synthase in Poplar (Populus spp.). Plant Molecular Biology. 73 (4-5)

Possell, M., Ryan, A., Vickers, CE., Mullineaux, PM. and Hewitt, CN., (2010). Effects of fosmidomycin on plant photosynthesis as measured by gas exchange and chlorophyll fluorescence. Photosynthesis Research. 104 (1)

BECHTOLD, U., LAWSON, T., MEJIA-CARRANZA, J., MEYER, RC., BROWN, IR., ALTMANN, T., TON, J. and MULLINEAUX, PM., (2010). Constitutive salicylic acid defences do not compromise seed yield, drought tolerance and water productivity in the Arabidopsis accession C24. Plant, Cell & Environment. 33 (11)

ESCOBAR, C., GARCÍA, A., ARISTIZÁBAL, F., PORTILLO, M., HERREROS, E., MUNOZ-MARTÍN, MA., GRUNDLER, F., MULLINEAUX, PM. and FENOLL, C., (2010). Activation of geminivirus V-sense promoters in roots is restricted to nematode feeding sites. Molecular Plant Pathology. 11 (3)

Galvez-Valdivieso, G., Fryer, MJ., Lawson, T., Slattery, K., Truman, W., Smirnoff, N., Asami, T., Davies, WJ., Jones, AM., Baker, NR. and Mullineaux, PM., (2009). The High Light Response in Arabidopsis Involves ABA Signaling between Vascular and Bundle Sheath Cells. THE PLANT CELL ONLINE. 21 (7)

Chang, CC., Slesak, I., Jorda, L., Sotnikov, A., Melzer, M., Miszalski, Z., Mullineaux, PM., Parker, JE., Karpinska, B. and Karpinski, S., (2009). Arabidopsis Chloroplastic Glutathione Peroxidases Play a Role in Cross Talk between Photooxidative Stress and Immune Responses. PLANT PHYSIOLOGY. 150 (2)

Bechtold, U., Rabbani, N., Mullineaux, PM. and Thornalley, PJ., (2009). Quantitative measurement of specific biomarkers for protein oxidation, nitration and glycation in Arabidopsis leaves. The Plant Journal. 59 (4)

VICKERS, CE., POSSELL, M., COJOCARIU, CI., VELIKOVA, VB., LAOTHAWORNKITKUL, J., RYAN, A., MULLINEAUX, PM. and NICHOLAS HEWITT, C., (2009). Isoprene synthesis protects transgenic tobacco plants from oxidative stress. Plant, Cell & Environment. 32 (5)

Driever, SM., Fryer, MJ., Mullineaux, PM. and Baker, NR., (2009). Imaging of reactive oxygen species in vivo.. Methods in molecular biology (Clifton, N.J.). 479

Muhlenbock, P., Szechynska-Hebda, M., Plaszczyca, M., Baudo, M., Mateo, A., Mullineaux, PM., Parker, JE., Karpinska, B. and Karpinski, S., (2008). Chloroplast Signaling and LESION SIMULATING DISEASE1 Regulate Crosstalk between Light Acclimation and Immunity in Arabidopsis. THE PLANT CELL ONLINE. 20 (9)

Morison, J., Baker, N., Mullineaux, P. and Davies, W., (2008). Improving water use in crop production. Philosophical Transactions of the Royal Society B: Biological Sciences. 363 (1491)

Charlton, AJ., Donarski, JA., Harrison, M., Jones, SA., Godward, J., Oehlschlager, S., Arques, JL., Ambrose, M., Chinoy, C., Mullineaux, PM. and Domoney, C., (2008). Responses of the pea (Pisum sativum L.) leaf metabolome to drought stress assessed by nuclear magnetic resonance spectroscopy. Metabolomics. 4 (4)

Illingworth, CJ., Parkes, KE., Snell, CR., Mullineaux, PM. and Reynolds, CA., (2008). Criteria for confirming sequence periodicity identified by Fourier transform analysis: Application to GCR2, a candidate plant GPCR?. Biophysical Chemistry. 133 (1-3)

Mühlenbock, P., Szechyńska-Hebda, M., Płaszczyca, M., Baudo, M., Mateo, A., Mullineaux, PM., Parker, JE., Karpińska, B. and Karpiński, S., (2008). Chloroplast signaling and Lesion Simulating Disease1 regulate crosstalk between light acclimation and immunity in Arabidopsis (Plant Cell (2008) 20, (2339-2356)). Plant Cell. 20 (12)

Bechtold, U., Richard, O., Zamboni, A., Gapper, C., Geisler, M., Pogson, B., Karpinski, S. and Mullineaux, PM., (2008). Impact of chloroplastic- and extracellular-sourced ROS on high light-responsive gene expression in Arabidopsis. Journal of Experimental Botany. 59 (2)

LAOTHAWORNKITKUL, J., PAUL, ND., VICKERS, CE., POSSELL, M., TAYLOR, JE., MULLINEAUX, PM. and HEWITT, CN., (2008). Isoprene emissions influence herbivore feeding decisions. Plant, Cell & Environment. 31 (10)

Laothawornkitkul, J., Paul, ND., Vickers, CE., Possell, M., Mullineaux, PM., Hewitt, CN. and Taylor, JE., (2008). The role of isoprene in insect herbivory. Plant Signaling & Behavior. 3 (12)

OVERMYER, K., KOLLIST, H., TUOMINEN, H., BETZ, C., LANGEBARTELS, C., WINGSLE, G., KANGASJÄRVI, S., BRADER, G., MULLINEAUX, P. and KANGASJÄRVI, J., (2008). Complex phenotypic profiles leading to ozone sensitivity in Arabidopsis thaliana mutants. Plant, Cell & Environment. 31 (9)

Vohra, S., Chintapalli, S., Illingworth, C., Reeves, P., Mullineaux, P., Clark, H., Dean, M., Upton, G. and Reynolds, C., (2007). Computational studies of Family A and Family B GPCRs. Biochemical Society Transactions. 35 (4)

Vickers, CE., Schenk, PM., Li, D., Mullineaux, PM. and Gresshoff, PM., (2007). pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in plants. Biotechnology Letters. 29 (11)

Mullineaux, PM., (2006). Spatial Dependence for Hydrogen Peroxide-Directed Signaling in Light-Stressed Plants. PLANT PHYSIOLOGY. 141 (2)

Flors, C., (2006). Imaging the production of singlet oxygen in vivo using a new fluorescent sensor, Singlet Oxygen Sensor Green(R). Journal of Experimental Botany. 57 (8)

Volkov, RA., Panchuk, II., Mullineaux, PM. and Schöffl, F., (2006). Heat stress-induced H2O2 is required for effective expression of heat shock genes in Arabidopsis. Plant Molecular Biology. 61 (4-5)

ROSSEL, JB., WALTER, PB., HENDRICKSON, L., CHOW, WS., POOLE, A., MULLINEAUX, PM. and POGSON, BJ., (2006). A mutation affecting ASCORBATE PEROXIDASE 2 gene expression reveals a link between responses to high light and drought tolerance. Plant, Cell and Environment. 29 (2)

Hernandez, JA., Escobar, C., Creissen, G. and Mullineaux, PM., (2006). Antioxidant enzyme induction in pea plants under high irradiance. Biologia Plantarum. 50 (3)

Carvalho, L., Vilela, B., Vidigal, P., Mullineaux, P. and Amâncio, S., (2006). Activation of the Ascorbate‐Glutathione Cycle Is an Early Response of Micropropagated Vitis vinifera L. Explants Transferred to ex Vitro. International Journal of Plant Sciences. 167 (4)

Mullineaux, PM. and Rausch, T., (2005). Glutathione, photosynthesis and the redox regulation of stress-responsive gene expression. Photosynthesis Research. 86 (3)

BECHTOLD, U., KARPINSKI, S. and MULLINEAUX, PM., (2005). The influence of the light environment and photosynthesis on oxidative signalling responses in plant-biotrophic pathogen interactions. Plant, Cell and Environment. 28 (8)

Carvalho, L., Vidigal, P., Vilela, B., Mullineaux, P. and Amâncio, S., (2005). MONITORING THE RESPONSE OF GRAPEVINE PLANTLETS TO EXCESS LIGHT BY REAL-TIME PCR. Acta Horticulturae. 689 (689)

Mateo, A., (2004). LESION SIMULATING DISEASE 1 Is Required for Acclimation to Conditions That Promote Excess Excitation Energy. PLANT PHYSIOLOGY. 136 (1)

Chang, CC-C., Ball, L., Fryer, MJ., Baker, NR., Karpinski, S. and Mullineaux, PM., (2004). Induction of ASCORBATE PEROXIDASE 2 expression in wounded Arabidopsis leaves does not involve known wound-signalling pathways but is associated with changes in photosynthesis. The Plant Journal. 38 (3)

Charlton, A., Allnutt, T., Holmes, S., Chisholm, J., Bean, S., Ellis, N., Mullineaux, P. and Oehlschlager, S., (2004). NMR profiling of transgenic peas. Plant Biotechnology Journal. 2 (1)

Asai, N., Matsuyama, T., Tamaoki, M., Nakajima, N., Kubo, A., Aono, M., Kato, T., Tabata, S., Shirano, Y., Shibata, D., Hayashi, H., Mullineaux, PM. and Saji, H., (2004). Compensation for lack of a cytosolic ascorbate peroxidase in an Arabidopsis mutant by activation of multiple antioxidative systems. Plant Science. 166 (6)

Bechtold, U., (2004). Arabidopsis Peptide Methionine Sulfoxide Reductase2 Prevents Cellular Oxidative Damage in Long Nights. THE PLANT CELL ONLINE. 16 (4)

Hernández, JA., Escobar, C., Creissen, G. and Mullineaux, PM., (2004). Role of hydrogen peroxide and the redox state of ascorbate in the induction of antioxidant enzymes in pea leaves under excess light stress. Functional Plant Biology. 31 (4)

Ball, L., (2004). Evidence for a Direct Link between Glutathione Biosynthesis and Stress Defense Gene Expression in Arabidopsis. THE PLANT CELL ONLINE. 16 (9)

Fryer, MJ., Ball, L., Oxborough, K., Karpinski, S., Mullineaux, PM. and Baker, NR., (2003). Control of Ascorbate Peroxidase 2 expression by hydrogen peroxide and leaf water status during excess light stress reveals a functional organisation of Arabidopsis leaves. The Plant Journal. 33 (4)

Karpinski, S., Gabrys, H., Mateo, A., Karpinska, B. and Mullineaux, PM., (2003). Light perception in plant disease defence signalling. Current Opinion in Plant Biology. 6 (4)

Muñoz-Martín, A., Collin, S., Herreros, E., Mullineaux, PM., Fernández-Lobato, M. and Fenoll, C., (2003). Regulation of MSV and WDV virion-sense promoters by WDV nonstructural proteins: a role for their retinoblastoma protein-binding motifs. Virology. 306 (2)

Escobar, C., Hernández, LE., Jiménez, A., Creissen, G., Ruiz, MT. and Mullineaux, PM., (2003). Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X. Plant Cell Reports. 21 (7)

Fryer, MJ., (2002). Imaging of photo-oxidative stress responses in leaves. Journal of Experimental Botany. 53 (372)

Mullineaux, P. and Karpinski, S., (2002). Signal transduction in response to excess light: getting out of the chloroplast. Current Opinion in Plant Biology. 5 (1)

Jimenez, A., Creissen, G., Kular, B., Firmin, J., Robinson, S., Verhoeyen, M. and Mullineaux, P., (2002). Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening. Planta. 214 (5)

Cleary, SP., Tan, F-C., Nakrieko, K-A., Thompson, SJ., Mullineaux, PM., Creissen, GP., von Stedingk, E., Glaser, E., Smith, AG. and Robinson, C., (2002). Isolated Plant Mitochondria Import Chloroplast Precursor Proteins in Vitro with the Same Efficiency as Chloroplasts. Journal of Biological Chemistry. 277 (7)

Pastori, GM., Mullineaux, PM. and Foyer, CH., (2000). Post-Transcriptional Regulation Prevents Accumulation of Glutathione Reductase Protein and Activity in the Bundle Sheath Cells of Maize. Plant Physiology. 122 (3)

Hellens, RP., Anne Edwards, E., Leyland, NR., Bean, S. and Mullineaux, PM., (2000). pGreen: A versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation. Plant Molecular Biology. 42 (6)

Dry, I., Krake, L., Mullineaux, P. and Rezaian, A., (2000). Regulation of Tomato Leaf Curl Viral Gene Expression in Host Tissues. Molecular Plant-Microbe Interactions. 13 (5)

Mullineaux, P., Ball, L., Escobar, C., Karpinska, B., Creissen, G. and Karpinski, S., (2000). Are diverse signalling pathways integrated in the regulation of Arabidopsis antioxidant defence gene expression in response to excess excitation energy?. Philosophical Transactions of the Royal Society B: Biological Sciences. 355 (1402)

Hernandez, JA., Jimenez, A., Mullineaux, P. and Sevilia, F., (2000). Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences. Plant, Cell and Environment. 23 (8)

Hellens, R., Mullineaux, P. and Klee, H., (2000). A guide to Agrobacterium binary Ti vectors. Trends in Plant Science. 5 (10)

Stevens, RG., Creissen, GP. and Mullineaux, PM., (2000). Characterisation of pea cytosolic glutathione reductase expressed in transgenic tobacco. Planta. 211 (4)

Mullineaux, P. and Yamasaki, H., (2000). Nitrite-dependent nitric oxide production pathway: implications for involvement of active nitrogen species in photoinhibition in vivo - Discussion. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES. 355 (1402)

Gooding, P., (1999). Plant cell-directed control of virion sense gene expression in wheat dwarf virus. Nucleic Acids Research. 27 (7)

Creissen, G., (1999). Elevated Glutathione Biosynthetic Capacity in the Chloroplasts of Transgenic Tobacco Plants Paradoxically Causes Increased Oxidative Stress. THE PLANT CELL ONLINE. 11 (7)

Karpinski, S., (1999). Systemic Signaling and Acclimation in Response to Excess Excitation Energy in Arabidopsis. Science. 284 (5414)

Foyer, CH. and Mullineaux, PM., (1998). The presence of dehydroascorbate and dehydroascorbate reductase in plant tissues. FEBS Letters. 425 (3)

Mullineaux, PM., Karpinski, S., Jimenez, A., Cleary, SP., Robinson, C. and Creissen, GP., (1998). Identification of cDNAS encoding plastid-targeted glutathione peroxidase. The Plant Journal. 13 (3)

Bean, SJ., Gooding, PS., Mullincaux, PM. and Davies, DR., (1997). A simple system for pea transformation. Plant Cell Reports. 16 (8)

Karpinski, S., (1997). Photosynthetic Electron Transport Regulates the Expression of Cytosolic Ascorbate Peroxidase Genes in Arabidopsis during Excess Light Stress. THE PLANT CELL ONLINE. 9 (4)

Stevens, RG., Creissen, GP. and Mullineaux, PM., (1997). Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress. Plant Molecular Biology. 35 (5)

Bean, SJ., Gooding, PS., Mullineaux, PM. and Davies, DR., (1997). A simple system for pea transformation. Plant Cell Reports. 16 (8)

Mullineaux, PM. and Creissen, GP., (1996). Opportunities for the genetic manipulation of antioxidants in plant foods. Biochemical Society Transactions. 24 (3)

COLLIN, S., FERNÁNDEZ-LOBATO, M., GOODING, PS., MULLINEAUX, PM. and FENOLL, C., (1996). The Two Nonstructural Proteins from Wheat Dwarf Virus Involved in Viral Gene Expression and Replication Are Retinoblastoma-Binding Proteins. Virology. 219 (1)

Santos, M., Gousseau, H., Lister, C., Foyer, C., Creissen, G. and Mullineaux, P., (1996). Cytosolic ascorbate peroxidase from Arabidopsis thaliana L. is encoded by a small multigene family. Planta. 198 (1)

Barnes, JD., Percy, KE., Paul, ND., Jones, P., McLaughlin, CK., Mullineaux, PM., Creissen, G. and Wellburn, AR., (1996). The influence of UV-B radiation on the physicochemical nature of tobacco ( Nicotiana tabacum L.) leaf surfaces. Journal of Experimental Botany. 47 (1)

Barnes, JD., Percy, KE., Paul, ND., Jones, P., McLaughlin, CK., Mullineaux, PM., Creissen, G. and Wellburn, AR., (1996). The influence of UV-B radiation on the physicochemical nature of tobacco (Nicotiana tabacum L.) leaf surfaces. Journal of Experimental Botany. 47 (294)

Mullineaux, P., Enard, C., Hellens, R. and Creissen, G., (1996). Characterisation of a glutathione reductase gene and its genetic locus from pea (Pisum sativum L.). Planta. 200 (2)

Broadbent, P., Creissen, G., Kular, B., Wellburn, A. and Mullineaux, P., (1995). Oxidative stress responses in transgenic tobacco containing altered levels of glutathione reductase activity. The Plant Journal. 8 (2)

Creissen, G. and Mullineaux, P., (1995). Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme. Planta. 197 (2)

Creissen, G., Reynolds, H., Xue, Y. and Mullineaux, P., (1995). Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco. The Plant Journal. 8 (2)

Forster, C., Arthur, E., Crespi, S., Hobbs, SLA., Mullineaux, P. and Casey, R., (1994). Isolation of a pea (Pisum sativum) seed lipoxygenase promoter by inverse polymerase chain reaction and characterization of its expression in transgenic tobacco. Plant Molecular Biology. 26 (1)

Broadbent, P., Creissen, G., Wellburn, FAM., Mullineaux, PM. and Wellburn, AR., (1994). Biochemical effects of tropospheric ozone in transgenic plants. Biochemical Society Transactions. 22 (4)

Chen, DF., Dale, PJ., Heslop-Harrison, JS., Snape, JW., Harwood, W., Bean, S. and Mullineaux, PM., (1994). Stability of transgenes and presence of N6 methyladenine DNA in transformed wheat cells. The Plant Journal. 5 (3)

Davies, D., Hamilton, J. and Mullineaux, P., (1993). Transformation of peas. Plant Cell Reports. 12 (3)

Hassan, MA., Swartz, HJ., Inamine, G. and Mullineaux, P., (1993). Agrobacterium tumefaciens-mediated transformation of severalRubus genotypes and recovery of transformed plants. Plant Cell, Tissue and Organ Culture. 33 (1)

Dry, IB., Rigden, JE., Krake, LR., Mullineaux, PM. and Rezaian, MA., (1993). Nucleotide sequence and genome organization of tomato leaf curl geminivirus. Journal of General Virology. 74 (1)

Mullineaux, PM., Rigden, JE., Dry, IB., Krake, LR. and Rezaian, M., (1993). Mapping of the Polycistronic RNAs of Tomato Leaf Curl Geminivirus. Virology. 193 (1)

Rigden, JE., Dry, IB., Mullineaux, PM. and Rezaian, M., (1993). Mutagenesis of the Virion-Sense Open Reading Frames of Tomato Leaf Curl Geminivirus. Virology. 193 (2)

Edwards, E., Enard, C., Creissen, G. and Mullineaux, P., (1993). Synthesis and properties of glutathione reductase in stressed peas. Planta. 192 (1)

Accotto, G-P., Mullineaux, P., Brown, S. and Marie, D., (1993). Digitaria Streak Geminivirus Replicative Forms Are Abundant in S-Phase Nuclei of Infected Cells. Virology. 195 (1)

Hofer, J., (1992). Coordinate Regulation of Replication and Virion Sense Gene Expression in Wheat Dwarf Virus. THE PLANT CELL ONLINE. 4 (2)

Guerineau, F., Lucy, A. and Mullineaux, P., (1992). Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts. Plant Molecular Biology. 18 (4)


Creissen, G., Edwards, EA., Enard, C., Wellburn, A. and Mullineaux, P., (1992). Molecular characterization of glutathione reductase cDNAs from pea (Pisum sativum L.). The Plant Journal. 2 (1)

Guerineau, F., Brooks, L. and Mullineaux, P., (1991). Effect of deletions in the cauliflower mosaic virus polyadenylation sequence on the choice of the polyadenylation sites in tobacco protoplasts. MGG Molecular & General Genetics. 226-226 (1-2)

Dekker, EL., Woolston, CJ., Xue, Y., Cox, B. and Mullineaux, PM., (1991). Transcript mapping reveals different expression strategies for the bicistronic RNAs of the geminivirus wheat dwarf virus. Nucleic Acids Research. 19 (15)

Guerineau, F., Brooks, L., Meadows, J., Lucy, A., Robinson, C. and Mullineaux, P., (1990). Sulfonamide resistance gene for plant transformation. Plant Molecular Biology. 15 (1)

Edwards, E., Rawsthorne, S. and Mullineaux, P., (1990). Subcellular distribution of multiple forms of glutathione reductase in leaves of pea (Pisum sativum L.). Planta. 180 (2)

Mullineaux, PM., Guerineau, F. and Accotto, G-P., (1990). Processing of complementary sense RNAs of Digitaria streak virus in its host and in transgenic tobacco. Nucleic Acids Research. 18 (24)

Kunert, K., Cresswell, C., Schmidt, A., Mullineaux, P. and Foyer, C., (1990). Variations in the activity of glutathione reductase and the cellular glutathione content in relation to sensitivity to methylviologen in Escherichia coli. Archives of Biochemistry and Biophysics. 282 (2)

Creissen, G., Smith, C., Francis, R., Reynolds, H. and Mullineaux, P., (1990). Agrobacterium ? and microprojectile ? mediated viral DNA delivery into barley microspore-derived cultures. Plant Cell Reports. 8 (11)

Guerineau, F., Brooks, L. and Mullineaux, P., (1990). Expression of the sulfonamide resistance gene from plasmid R46. Plasmid. 23 (1)

Guerineau, F. and Mullineaux, P., (1989). Nucleotide sequence of the sulfonamide resistance gene from plasmid R46. Nucleic Acids Research. 17 (11)

Dale, P., Marks, M., Brown, M., Woolston, C., Gunn, H., Mullineaux, P., Lewis, D., Kemp, J., Chen, D., Gilmour, D. and Flavell, R., (1989). Agroinfection of wheat: Inoculation of in vitro grown seedlings and embryos. Plant Science. 63 (2)

Woolston, CJ., Reynolds, HV., Stacey, NJ. and Mullineaux, P., (1989). Replication of wheat dwarf virus DNA in protoplasts and analysis of coat protein mutants in protoplasts and plants. Nucleic Acids Research. 17 (15)

Accotto, GP., Donson, J. and Mullineaux, PM., (1989). Mapping of Digitaria streak virus transcripts reveals different RNA species from the same transcription unit.. EMBO Journal. 8 (4)

Donson, J., Gunn, HV., Woolston, CJ., Pinner, MS., Boulton, MI., Mullineaux, PM. and Davies, JW., (1988). Agrobacterium-mediated infectivity of cloned digitaria streak virus DNA. Virology. 162 (1)

Mullineaux, PM., Boulton, MI., Bowyer, P., van der Vlugt, R., Marks, M., Donson, J. and Davies, JW., (1988). Detection of a non-structural protein of M r 11 000 encoded by the virion DNA of maize streak virus. Plant Molecular Biology. 11 (1)

Guerineau, F., Woolston, S., Brooks, L. and Mullineaux, P., (1988). An expression cassette for targeting foreign proteins into chloroplasts. Nucleic Acids Research. 16 (23)

Woolston, CJ., Barker, R., Gunn, H., Boulton, MI. and Mullineaux, PM., (1988). Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA. Plant Molecular Biology. 11 (1)

Harker, CL., Mullineaux, PM., Bryant, JA. and Maule, AJ., (1987). Detection of CaMV gene I and gene VI protein products in vivo using antisera raised to COOH-terminal ?-galactosidase fusion proteins. Plant Molecular Biology. 8 (3)

Davies, JW., Stanley, J., Donson, J., Mullineaux, PM. and Boulton, MI., (1987). Structure and replication of geminivirus genomes.. Journal of cell science. Supplement. 7

Donson, J., Accotto, GP., Boulton, MI., Mullineaux, PM. and Davies, JW., (1987). The nucleotide sequence of a geminivirus from Digitaria sanguinalis. Virology. 161 (1)

Morris-Krsinich, BA., Mullineaux, PM., Donson, J., Boulton, MI., Markham, PG., Short, MN. and Davies, JW., (1985). Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene. Nucleic Acids Research. 13 (20)

Mullineaux, PM., Donson, J., Stanley, J., Boulton, MI., Morris-Krsinich, BAM., Markham, PG. and Davies, JW., (1985). Computer analysis identifies sequence homologies between potential gene products of Maize Streak Virus and those of Cassava Latent Virus and Tomato Golden Mosaic Virus. Plant Molecular Biology. 5 (2)

Mullineaux, P. and Willetts, N., (1985). Promoters in the transfer region of plasmid F.. Basic life sciences. 30

Mullineaux, PM., Donson, J., Morris-Krsinich, BA., Boulton, MI. and Davies, JW., (1984). The nucleotide sequence of maize streak virus DNA.. EMBO Journal. 3 (13)

Donson, J., Morris-Krsinich, BA., Mullineaux, PM., Boulton, MI. and Davies, JW., (1984). A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated.. EMBO Journal. 3 (13)


Mullineaux, PM., Chaplin, AE. and Gallon, JR., (1983). Synthesis of nitrogenase in the cyanobacterium Gloeothece (Gloeocapsa) sp. CCAP 1430/3. Journal of General Microbiology. 129 (6)


Thomas, JH., Mullineaux, PM. and Cronshaw, AD., (1982). The effects of structural analogues of amino acids on ammonium assimilation and acetylene reduction (nitrogen fixation) in Gloeocapsa (gloeothece) sp. CCAP 1430/3. Journal of General Microbiology. 128 (4)

Millineaux, PM., Gallon, JR. and Chaplin, AE., (1981). Acetylene reduction (nitrogen fixation) by cyanobacteria grown under alternating light-dark cycles. FEMS Microbiology Letters. 10 (3)



Mullineaux, PM., Chaplin, AE. and Gallon, JR., (1980). Effects of a light to dark transition on carbon reserves, nitrogen fixation and ATP concentrations in cultures of Gleocapsa (Gloeothece) sp. 1430/3. Journal of General Microbiology. 120 (1)

MULLINEAUX, PM., CHAPLIN, AE. and GALLON, JR., (1979). The Short-Term Effect of Transfer from Light to Darkness on Acetylene Reduction (Nitrogen Fixation) by Cultures of Gloeocapsa sp. 1430/3. Biochemical Society Transactions. 7 (6)

Chapters (3)

Exposito-Rodriguez, M., Laissue, PP., Littlejohn, GR., Smirnoff, N. and Mullineaux, PM., (2013). The Use of HyPer to Examine Spatial and Temporal Changes in H₂O₂ in High Light-Exposed Plants. In: Methods in Enzymology (Vol. 527). Editors: Cadenas, E. and Packer, L., . Elsevier. 185- 201. 9780124058828

Mullineaux, P., (2012). Plant transformation and expression vectors. In: Plant Molecular Biology LabFax: A Volume in LabFax. 121- 147. 9780080917535

Mullineaux, PM. and Lawson, T., (2009). Measuring redox changes in vivo in leaves: prospects and technical challenges.. In: Redox-Mediated Signal Transduction: Methods and Protocols. 65- 75. 978-1617378027

Conferences (24)

Carvalho, L., Vilela, B., Mullineaux, PM. and Amancio, S., (2007). The role of peroxiredoxins in the response of grapevine to light stress

Vohra, S., Chintapalli, SV., Illingworth, CJR., Reeves, PJ., Mullineaux, PM., Clark, HSX., Dean, MK., Upton, GJG. and Reynolds, CA., (2007). Computational studies of Family A and Family B GPCRs

Cojocariu, C., Vickers, C., Velikova, V., Hewitt, N. and Mullineaux, P., (2007). Isoprene-induced protection in tobacco plants exposed to elevated ozone concentration

Mullineaux, P., (2007). Spatial dependence for (HO2)-O-2-directed signalling in Arabidopsis leaves subject to high light.

Mullineaux, P., Baker, N., Bechtold, U., Fryer, M. and Davies, W., (2007). Spatial dependence for H2O2-directed signalling in Arabidopsis leaves

Mateo, A., (2006). Controlled levels of salicylic acid are required for optimal photosynthesis and redox homeostasis

Baker, NR., Fryer, MJ., Morison, JIL., Davies, WJ. and Mullineaux, PM., (2005). Interactive roles for light, reactive oxygen species and leaf water status in signalling responses for gene expression during photo-oxidative stress

Funck, D., Matheo, A., Mullineaux, P. and Karpinski, S., (2004). EXCESS LIGHT SENSITIVITY OF ARABIDOPSIS MUTANT cpr6-1

Bechtold, U., Leyland, NR. and Mullineaux, PM., (2003). Does elevated foliar glutathione alter responses to biotic and abiotic stress?

Mullineaux, P., Ball, L., Karpinski, B., Fryer, M., Leyland, N., Baker, N. and Karpinski, S., (2003). Foliar responses to excess light stress in Arabidopsis mediated by the antioxidant glutathione.

Mullineaux, P., Ball, L., Karpinski, B., Fryer, M., Leyland, N., Baker, N. and Karpinski, S., (2003). Foliar responses to excess light stress and pathogens in Arabidopsis mediated by the antioxidant glutathione

Asai, N., Matsuyama, T., Tamaoki, M., Nakajima, N., Kubo, A., Aono, M., Kato, T., Tabata, S., Shirano, Y., Shibata, D., Hayashi, H., Mullineaux, PM. and Saji, H., (2002). Characterization of an Arabidopsis mutant deficient in a cytosolic ascorbate peroxidase APX1

Pastori, G., Foyer, CH. and Mullineaux, P., (2000). Low temperature‐induced changes in the distribution of H2O2 and antioxidants between the bundle sheath and mesophyll cells of maize leaves

Hellens, R., Joyce, N. and Mullineaux, P., (1999). A new and versatile Agrobacterium-based plant transformation vector

Mullineaux, P. and Creissen, G., (1999). Manipulating oxidative stress responses using transgenic plants: Successes and dangers

Lea, PJ., Wellburn, FAM., Wellburn, AR., Creissen, GP. and Mullineaux, PM., (1998). Use of transgenic plants in the assessment of responses to atmospheric pollutants

Wellburn, FAM., Creissen, GP., Lake, JA., Mullineaux, PM. and Wellburn, AR., (1998). Tolerance to atmospheric ozone in transgenic tobacco over-expressing glutathione synthetase in plastids

Karpinski, S., Karpinska, B., Reynolds, H., Creissen, G., Wingsle, G. and Mullineaux, P., (1998). Systemic sensing of light in plants; A key regulatory role of photosynthetic electron transport

Wellburn, AR., Wellburn, FAM., Creissen, GP. and Mullineaux, PM., (1997). Responses and consequences arising from the over-expression of glutathione levels in transgenic tobacco.

Creissen, G., Broadbent, P., Stevens, R., Wellburn, AR. and Mullineaux, P., (1996). Manipulation of glutathione metabolism in transgenic plants

Harwood, WA., Bean, SJ., Chen, DF., Mullineaux, PM. and Snape, JW., (1995). Transformation studies in Hordeum vulgare using a highly regenerable microspore system

Creissen, GP., Broadbent, P., Kular, B., Reynolds, H., Wellburn, AR. and Mullineaux, PM., (1994). Manipulation of glutathione reductase in transgenic plants: implications for plants' responses to environmental stress

Mullineaux, P., Creissen, G., Broadbent, P., Reynolds, H., Kular, B. and Wellburn, A., (1994). Elucidation of the role of glutathione reductase using transgenic plants


Grants and funding


Bioimaging of dehydroascorbate and (phospho)lipid hydroperoxides: The development of fluorescent protein biosensors

Biotechnology & Biology Science Res.Council


India - Transfer of Regulatory Genes from Arabidopsis to Indian Mustard

Biotechnology & Biology Science Res.Council


Development of plant-based hydrogen peroxide YFP nanosensors

Biotechnology & Biology Science Res.Council

Environmental meta-genomics and biotechnology of Rhazya

King Abdulaziz University


European Commission


Exploitation of Transcription Factor from Arabidopsis

Biotechnology & Biology Science Res.Council

+44 (0) 1206 872118


3SW.4.34, Colchester Campus

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