From 3D chromatin organisation to transcription factor binding in Drosophila
13:00 - 14:00
Dr Radu Zabet (University of Essex)
Lectures, talks and seminars
Life Sciences, School of
Dr Patrick Varga-Weisz firstname.lastname@example.org
This event has been postponed. We hope to arrange a new date for it in due course.
Compartmentalisation of the genome as topologically associating domains (TADs) was shown to have regulatory role in development and cellular functioning, but the mechanism involved in TAD establishment is still unclear.
We generated the first high-resolution contact map of Drosophila melanogaster neuronal cells (BG3) and identified different classes of TADs by comparing this to the genome 3D organisation in embryonic cells (Kc167).
In particular, we observed changes in genome organisation in differentiated neuronal cells, consisting mainly of enhanced long-range interactions and we show that the rich repertoire of architectural proteins in Drosophila might have a tissue specific role in the chromatin organisation.
Our results indicate that TADs are dynamic across developmental stages and reflect the interplay between architectural proteins, transcriptional states and enhancer activities.