Aurodox – A Type III Secretion System inhibitor from Streptomyces
Aurodox is an elfamycin-like specialised metabolite produced by the soil bacterium Streptomyces goldiniensis, that in addition to blocking translation can also inhibit the Type III Secretion System.
To gain a better understanding of its mechanism of action and to assess the utility of this compound as an anti-virulence molecule we undertook a multidisciplinary approach to understanding aurodox.
We have investigated the mechanism of action of aurodox in Enteropathogenic and Enterohemorrhagic Escherichia coli and shown that it acts through inhibition of the master regulator of the locus of enterocyte effacement and in parallel we investigated the biosynthesis of aurodox by Streptomyces goldiniensis, identifying the biosynthetic gene cluster for aurodox and dissecting its biosynthetic pathway.