The current thermodynamic description of B-form DNA stability is seriously in error in two critical aspects. 1) The enthalpy and entropy of unfolding are shown to be temperature dependent quantities. 2) The greater stabilizing effect of CG pairs does not come from their extra H-bond but from their lower entropy of melting.
A consequence of the new findings is that melting temperature calculations and secondary structure predictions need to use methods quite different from those currently employed.
Professor Colyn Crane-Robinson is an Emeritus Professor of the University of Portsmouth.