People

Professor Nelson Fernandez

Professor
School of Biological Sciences
Professor Nelson Fernandez
  • Email

  • Telephone

    +44 (0) 1206 873330

  • Location

    3SW.4.22, Colchester Campus

Profile

Biography

Career resume I did a BSc at Kings College (formerly Queen Elizabeth College, London), MSc at Brunel University (London) and PhD (Immunology) at the Royal London Hospital (London). I worked for several years in the field of transplantation immunology with the late Professor Hilliard Festenstein at the Royal London supported by the MRC. In 1990 I was appointed as a lecturer at the Department of Biological Sciences (University of Essex). Teaching Over the years I have supervised to completion a number of doctoral students. I currently supervise several PhD candidates. I also teach immunology for post-graduate courses at masters level for an MSc in Biotechnology and an MSc in Molecular Medicine I created the contents of two undergraduate modules, BS223 and BS326. I am the module organizer and teacher in both modules. The modules are: (1) Immunity in Health and Disease (BS223) for 2nd year BSc degrees and (2) Molecular and Developmental Immunology (BS326) for 3rd year BSc degrees. In 1999 I was awarded the Teaching and Learning Innovation Award by the University of the Essex. I also teach in the modules: Issues in Cell and Molecular Biology (BS304) and Molecular Mechanisms of Disease and Molecular Medicine (BS316). Wider Involvement I am currently Head of Molecular Medicine and director of the Cell Bioimaging facility at the University of Essex. In 1996 together with Professor G. Voissin (Paris) I participated in the foundation of the European Society of Reproduction Immunology (ESRI). In September 2005 we hosted the 3rd European Congress in reproductive immunology at Essex. Details of the ESRI web site can be viewed at: http://www.es4ri.eu/ In 2007, at the European Reproductive Congress in Germany, I was elected as member of the ESRI executive council. My main research interest resides in the investigation of the factors that control the cell-surface organization of immunoreceptors encoded by the Major Histocompatibility Complex (MHC). The MHC molecules are critical for the regulation of the immune response because they capture and display peptides, mainly those derived from pathogens to T lymphocytes. The MHC molecules are loaded with peptides in the cytoplasm and display these at the cell surface of subpopulations of cells, primarily antigen presenting cells. At the cell surface the MHC-peptide complex exist as non-covalently associated pair of sub-units. A subunit with a bound peptide functions as a ligand for antigen-specific T cell receptors on T lymphocytes. These are the cells, which control the specific adaptive responses of the immune system. As integral membrane proteins the MHC molecules have the capacity to redistribute in the membrane. Such redistribution is under the influence of constraints imposed by the cytoskeleton and the modulatory activity exerted upon receptor-density by, for example cytokines and other factors. Because of the lipid environment and the fluidity of the cell membrane the MHC molecules are potentially mobile (by lateral diffusion). They can enter into associations with each other (i.e., they can dimerize) or they can associate with other co-stimulatory molecules. A fundamental problem in immunobiology is to understand how MHC immunoreceptors and their corresponding subunits and associated structures interact at the cell surface. Further questions concern the nature of the changes that may be triggered, for example, in viral and bacterial infections and in autoimmunity. A hypothesis to be tested is that the MHC molecules are displayed in functional domains comprising single pairs or more than one pair of MHC subunits in close association. This pattern of cell-surface associations may reflect degrees of cellular activation or inhibition such as occur in anergy. We have been interested in testing these ideas by establishing novel and sensitive techniques that would allow us to study the behaviour (mobility and receptor associations) of the MHC molecules at the cell-surface on single living cells. Our aim is to eventually relate this to the function of these molecules in normal and disease satates. We are currently using molecular probes (e.g., Fab fragment of an antibody coupled to a fluorescent particle) and a single particle imaging system on a cooled slow-scan coupled charged device (CCD) digital camera. The system permits the resolution of immunological receptors at a nanometer-scale on single living cells in tissue culture. Thus, in essence, this technique permits the study of receptors in their native configuration. This approach obviates the use of conventional biochemical extraction procedures, traditionally used in immunology, such as the use of non-ionic or zwitterionic detergents that disrupt the cell membrane and hence affect protein-protein interactions. Using this experimental approach we have recently shown that a subset of the human MHC molecules, known as HLA DR, redistribute at the cell surface in a temperature-dependent equilibrium between single HLA DR heterodimers and dimers of heterodimers. The biological role of such dimer of dimers has not yet been established and is currently the subject of collaborative research with other groups in other universities. Interestingly the spatial resolution of receptor expression using the above approach has wider applicability in membrane immunology. It can provide information on the pattern of cell-surface organization of virtually any immunoreceptor in the normal and disease state. One such application has been found our current studies on the function of the MHC in development and differentiation. The hypothesis here is that the expression of MHC subsets is developmentally regulated, with selective sets of these molecules playing a role in embryo compaction and polarization. This hypothesis can be directly tested using single particle imaging on embryonic cells kept in vivo under culture conditions. In previous work here at Essex, has shown that in the mouse the mRNA MHC transcripts are expressed from one-cell after conception and prior to implantation of the embryo in the maternal uterus. Both maternally encoded and paternally encoded genes of the MHC are expressed in the one-cell embryo. The role of these molecules in the actual process of embryonic development remains to be elucidated. Projects currently in progress: The relationship between Major Histocompatibility Complex (MHC) Class II receptors and the membrane-bound Class-II associated invariant chain protein, CD74 and the macrophage-migration inhibitory factor, MIF. This project involves detection and quantification of receptor dimers and microclustering at the cell surface. The effects of reverse agonist peptides on the formation of protein dimers and aggregates are also studied. The cooperation of CD14, Toll-like receptors and other co-receptors in bacteria recognition by human monocytes. The link between poly (ADP-ribose) polymerases, PARP, and CD14 in human monocytes. The role of the human CTLA-4 molecules and other co-receptors; their expression on macrophages and characterisation on myeloid-derived cells. Proteomics of mouse abortogenic proteins. The ontogeny of MHC Class I receptors HLA-G (soluble and membrane-bound) in human early development. Role of glucocorticoid receptor isoforms in human rheumatoid arthritis and sensitivity to steroid treatment. Other recent projects: Isolation, detection and PCR analysis of HLA genes from ancient human DNA from a Colchester archaeological site. Wellcome Trust-funded Art-Science project. Human Organ Transplantation: A Question of Heart? see the link 30856 UNI UECLAA Report Group members: Dr Ian Morrison (Cell Bioimaging Unit) Julie Arvidson (Technican in Cell and Molecular Biology) Essam Elzatma (PhD student) Mario Nebenfuehr (PhD student) Panagiotis Koumpouros (PhD student) Valerie Shaikly (PhD student) Ayesha Shakhawat (PhD student) Johana Martinez (MSc student by research) Asma Jabeen (PhD student) Jamila Alhoderi (PhD student) Kamaran Mohamad (MSc student by research ) Most recent PhD and MSc graduates: Madhaviah Sukadev, Srividya PhD (2008) Thesis title: Expression of alpha and beta isoforms of glucocorticoid receptor and their interactions with NF-kB in synovial fibroblasts cells. AlMubarak, Mohammed PhD (2008) Thesis title: Study of the relationship of MHC class II associated invariant chain, CD74, with the macrophage migration inhibitory factor, MIF, on human tumour cell lines. Mavrakos, Manos PhD (2007) Thesis title: Regulation of CD14 expression and cytokine production in LPS stimulated macrophages by Poly-ADP rybosylation. Julia Richardt PhD (external at the Robert Koch Institute, Germany) 2006 Thesis title: T cell epitope variation and cellular responses to autologous isolates in therapy-naive HIV infected individuals. Karakikes, Ioannis PhD (2004) Thesis title: Association of the invariant chain (CD74) and human leukocyte antigen (HLA-DR): a single molecule approach. Adewuyi, Damilola, MSc Biotech (2005, awarded distinction) Thesis title: Identification of novel biomarkers of recurrent spontaneous abortion: a proteomic approach using abortogenic proteomic models. Rajasagi, Mohini, MSc Biotech (2005, awarded distinction) Thesis title: Proteomic characterisation of lipid rafts in response to lipopolysaccharide stimulation. Vasundhara, Vishnoi, MSc Biotech (2005, awarded distinction) Thesis title: Inhibitory influence of lactoferrin on CD14 interaction with lipopolysaccharides. Experimental approaches: We employ a range of immunological assays, including single particle fluorescent imaging and confocal microscopy, proteomics including immunoprecipitations, chemical cross-linking, Two-Dimensional electrophoresis (non-equilibrium pH gradient electrophoresis, isoelectric focusing and immobilised pH gradient protocols), LC/mass spectrometry and surface plasmon resonance analysis with a Biacore instrument. Research Funding: MRC, BBSRC, The Wellcome Trust, the DAAD Anglo-German, EU, ARGC, Southen Hospital and the Research Promotion Fund of the University of Essex. Current Funding: The Wellcome Trust, ARGC and Southend Hospital. Environment: The immunological work at Essex involves collaboration with a number of research groups within the department including Professor Richard Cherry on the bioimaging front, Dr Metodi Metodiev (Proteomics), Dr Minnie O'Farrell (PARP studies), Professor Paul Thornalley (Molecular Medicine) and Chris Reynolds (Computational Bioinformatics). The Department provides a broad-based structure and a multidisciplinary environment, involving collaboration between chemists, biochemists and biologists. The immunology work have access to modern facilites; we are equipped with a BioRad confocal microscope, an ultrasensitive digital fluorescence imaging system, a FACS Calibre flow cytometer, several fluorimeters and fluorescence stopped-flow equipment, luminometers, an automated genetic analyser (ABI310) for DNA sequencing and fingerprinting, the Applied Biosystems ABI7000 (2203) real-time PCR system, various PCR thermocyclers, an oligonucleotide synthesiser, a gel documentation system, ultracentrifuges, a computerised ELISA machine, and a recently refurbished radioisotope suite. The Genomics and Proteomics Suite at Essex is equipped with facilities for mass-spectroscopy (analytical MS laboratories) with MALDI-TOF-(Bruker Daltonics Reflex 4), nano-LC-MS/MS (Bruker Daltonics Esquire HCT), LC-MS/MS (Waters Alliance LC and Micromass Quattro Ultima triple quadrupole MS), analytical and preparative HPLC and microarray work (Axon microarray reader and software, Agilent Bioanalyser).

Research and professional activities

Current research

The biochemistry and physiological role of protein-protein interactions of immunological receptors.

Response to infection by LPS and gram-negative bacteria and CD14 and TLR interactions;

Immunological aspects of reproductive immunology.

inhibitory loops via CTLA-4 expressed on monocytes.

Human autoimmunity and glucocorticoid receptors.

Publications

Journal articles (106)

Thaker, YR., Recino, A., Raab, M., Jabeen, A., Wallberg, M., Fernandez, N. and Rudd, CE., (2017). Activated Cdc42-associated kinase 1 (ACK1) binds the sterile α motif (SAM) domain of the adaptor SLP-76 and phosphorylates proximal tyrosines. Journal of Biological Chemistry. 292 (15), 6281-6290

Hakam, MS., Miranda-Sayago, JM., Hayrabedyan, S., Todorova, K., Spencer, PS., Jabeen, A., Barnea, ER. and Fernandez, N., (2017). PreImplantation Factor (PIF) promotes HLA-G, -E, -F, -C expression in JEG-3 choriocarcinoma cells and endogenous progesterone activity. Cellular Physiology and Biochemistry. 43 (6), 2277-2296

Al Ssadh, H., Spencer, PS., Alabdulmenaim, W., Alghamdi, R., Madar, IM., Miranda-Sayago, JM. and Fernandez, N., (2017). Measurements of heterotypic associations between cluster of differentiation CD74 and CD44 in human breast cancer-derived cells. Oncotarget. 8 (54), 92143-92156

Voong, CP., Spencer, PS., Navarrete, CV., Turner, D., Hayrabedyan, SB., Crummy, P., Holloway, E., Wilson, MT., Smith, PR. and Fernández, N., (2017). HLA-DR genotyping and mitochondrial DNA analysis reveal the presence of family burials in a fourth century Romano-British Christian cemetery. Frontiers in Genetics. 8 (DEC), 182-

Calix, RX., Ornaghi, S., Wilson, JH., Fernandez, N., Vialard, F., Barnea, ER. and Paidas, MJ., (2017). Prelmplantation Factor and Endocrinology of Implantation and Establishment of Early Pregnancy: A Contemporary View. Pediatric Endocrinology Reviews. 15 (2), 147-158

Todorova, K., Metodiev, MV., Metodieva, G., Mincheff, M., Fernández, N. and Hayrabedyan, S., (2017). Micro-RNA-204 Participates in TMPRSS2/ERG Regulation and Androgen Receptor Reprogramming in Prostate Cancer. Hormones and Cancer. 8 (1), 28-48

Calix, RX., Ornaghi, S., Wilson, JH., Fernandez, N., Vialard, F., Barnea, ER. and Paidas, MJ., (2017). Preimplantation factor and endocrinology of implantation and establishment of early pregnancy: A contemporary view. Pediatric Endocrinology Reviews. 15 (2), 147-158

Nikolaeva, MA., Babayan, AA., Stepanova, EO., Smolnikova, VY., Kalinina, EA., Fernández, N., Krechetova, LV., Vanko, LV. and Sukhikh, GT., (2016). The relationship of seminal transforming growth factor-β1 and interleukin-18 with reproductive success in women exposed to seminal plasma during IVF/ICSI treatment. Journal of Reproductive Immunology. 117, 45-51

Hayrabedyan, S., Todorova, K., Jabeen, A., Metodieva, G., Toshkov, S., Metodiev, MV., Mincheff, M. and Fernández, N., (2016). Sertoli cells have a functional NALP3 inflammasome that can modulate autophagy and cytokine production. Scientific Reports. 6 (1), 18896-

Amirfakhri, S., Salimi, A. and Fernandez, N., (2016). Effects of conditioned medium from breast cancer cells on Tlr2 expression in Nb4 cells. Asian Pacific Journal of Cancer Prevention. 16 (18), 8445-8450

Hayrabedyan, S., Todorova, K., Jabeen, A., Metodieva, G., Toshkov, S., Metodiev, M., Mincheff, M. and Fern�ndez, N., (2016). Sertoli cells have a functional NALP3 inflammasome that can modulate autophagy and cytokine production. Scientific Reports. 6 (18896)

Amirfakhri, S., Salimi, A. and Fern�ndez, N., (2016). Estudy the Effect of Breast Cancer on Tlr2 Expression in Nb4 Cell. Asian Pacific Journal of Cancer Prevention. 16 (18), 8445-8450

Barnea, ER., Hayrabedyan, S., Todorova, K., Almogi-Hazan, O., Or, R., Guingab, J., McElhinney, J., Fernandez, N. and Barder, T., (2016). PreImplantation factor (PIF*) regulates systemic immunity and targets protective regulatory and cytoskeleton proteins. Immunobiology. 221 (7), 778-793

Barnea, ER., Kirk, D., Todorova, K., McElhinney, J., Hayrabedyan, S. and Fernández, N., (2015). PIF direct immune regulation: Blocks mitogen-activated PBMCs proliferation, promotes TH2/TH1 bias, independent of Ca2+. Immunobiology. 220 (7), 865-875

Jabeen, A., Miranda-Sayago, JM., Obara, B., Spencer, PS., Dealtry, GB., Hayrabedyan, S., Shaikly, V., Laissue, PP. and Fernandez, N., (2013). Quantified Colocalization Reveals Heterotypic Histocompatibility Class I Antigen Associations on Trophoblast Cell Membranes: Relevance for Human Pregnancy1. Biology of Reproduction. 89 (4), 94-

Pérez, O., Romeu, B., Cabrera, O., González, E., Batista-Duharte, A., Labrada, A., Pérez, R., Reyes, LM., Ramírez, W., Sifontes, S., Fernández, N. and Lastre, M., (2013). Adjuvants are Key Factors for the Development of Future Vaccines: Lessons from the Finlay Adjuvant Platform. Frontiers in Immunology. 4 (DEC), 407-

Obara, B., Jabeen, A., Fernandez, N. and Laissue, PP., (2013). A novel method for quantified, superresolved, three-dimensional colocalisation of isotropic, fluorescent particles. Histochemistry and Cell Biology. 139 (3), 391-402

Todorova, K., Mincheff, M., Hayrabedyan, S., Mincheva, J., Zasheva, D., Kuzmanov, A. and Fernández, N., (2013). Fundamental Role of microRNAs in Androgen-Dependent Male Reproductive Biology and Prostate Cancerogenesis. American Journal of Reproductive Immunology. 69 (2), 100-104

P�rez, O., Romeu, B., Cabrera, O., Gonz�lez, E., Batista-Duharte, A., Labrada, A., P�rez, R., Reyes, LM., Ram�rez, W., Sifontes, S., Fern�ndez, N. and Lastre, M., (2013). Adjuvants are Key Factors for the Development of Future Vaccines: Lessons from the Finlay Adjuvant Platform.. Frontiers in immunology. 4, creators-Fern=E1ndez=3ANelson=3A=3A

Spencer, PS., Hakam, SM., Laissue, PP., Jabeen, A., Jain, P., Hayrabedyan, S., Todorova, K., Blanch, A., McElhinney, JM., Muhandiram, N., Alkhatib, S., Dealtry, GB., Miranda-Sayago, JM. and Fernández, N., (2012). Key Cellular Components and Interactive Histocompatibility Molecules Regulating Tolerance to the Fetal Allograft. American Journal of Reproductive Immunology. 68 (2), 95-99

Karakikes, I., Morrison, IEG., O'Toole, P., Metodieva, G., Navarrete, CV., Gomez, J., Miranda-Sayago, JM., Cherry, RJ., Metodiev, M. and Fernandez, N., (2012). Interaction of HLA-DR and CD74 at the cell surface of antigen-presenting cells by single particle image analysis. The FASEB Journal. 26 (12), 4886-4896

Greenwood, C., Metodieva, G., Al-Janabi, K., Lausen, B., Alldridge, L., Leng, L., Bucala, R., Fernandez, N. and Metodiev, MV., (2012). Stat1 and CD74 overexpression is co-dependent and linked to increased invasion and lymph node metastasis in triple-negative breast cancer. Journal of Proteomics. 75 (10), 3031-3040

Hayrabedyan, S., Todorova, K., Pashova, S., Mollova, M. and Fernández, N., (2012). Sertoli Cell Quiescence - New Insights. American Journal of Reproductive Immunology. 68 (6), 451-455

Kyurkchiev, S., Gandolfi, F., Hayrabedyan, S., Brevini, TAL., Dimitrov, R., Fitzgerald, JS., Jabeen, A., Mourdjeva, M., Photini, SM., Spencer, P., Fernández, N. and Markert, UR., (2012). Stem Cells in the Reproductive System. American Journal of Reproductive Immunology. 67 (6), 445-462

Jabeen, A., Alkhatib, S., Hicks, CE., Uddin, T., Hakam, SM., Jain, P. and Fern�ndez, N., (2012). Extra-villous trophoblast cytoskeleton deformation under co-infection with viral and bacterial agents. Journal of reproductive immunology. 94 (1), creators-Fern=E1ndez=3ANelson=3A=3A

Lee, C., Lam, KKW., Koistinen, H., Seppala, M., Kurpisz, M., Fern�ndez, N., Pang, RTK., Yeung, WSB. and Chiu, PCN., (2011). Glycodelin-A as a paracrine regulator in early pregnancy. Journal of reproductive immunology. 90 (1, SI), 29-34

Shaikly, V., Shakhawat, A., Withey, A., Morrison, I., Taranissi, M., Dealtry, GB., Jabeen, A., Cherry, R. and Fernández, N., (2010). Cell bio-imaging reveals co-expression of HLA-G and HLA-E in human preimplantation embryos. Reproductive BioMedicine Online. 20 (2), 223-233

Shakhawat, A., Shaikly, V., Elzatma, E., Mavrakos, E., Jabeen, A. and Fernández, N., (2010). Interaction between HLA-G and monocyte/macrophages in human pregnancy. Journal of Reproductive Immunology. 85 (1), 40-46

Shakhawat, A., Shaikly, VR. and Fern�ndez, N., (2010). Anti-inflammatory interleukin-4 (IL-4) cytokine regulates human leukocyte antigen-G expression in a trophoblast cell line. Journal of reproductive immunology. 86 (2, SI), creators-Fern=E1ndez=3ANelson=3A=3A

Shakhawat, A., Shaikly, V., Mavrakos, E. and Fern�ndez, N., (2009). The protective roles of non-classical MHC class Ib HLA-G antigen against maternal macrophages. Journal of reproductive immunology. 81 (2), creators-Fern=E1ndez=3ANelson=3A=3A

Shaikly, VR., Morrison, IEG., Taranissi, M., Noble, CV., Withey, AD., Cherry, RJ., Blois, SM. and Fernandez, N., (2008). Analysis of HLA-G in Maternal Plasma, Follicular Fluid, and Preimplantation Embryos Reveal an Asymmetric Pattern of Expression. The Journal of Immunology. 180 (6), 4330-4337

Blois, SM., Barrientos, G., Garcia, MG., Orsal, AS., Tometten, M., Cordo-Russo, RI., Klapp, BF., Santoni, A., Fernández, N., Terness, P. and Arck, PC., (2008). Interaction between dendritic cells and natural killer cells during pregnancy in mice. Journal of Molecular Medicine. 86 (7), 837-852

Krey, G., Frank, P., Shaikly, V., Barrientos, G., Cordo-Russo, R., Ringel, F., Moschansky, P., Chernukhin, IV., Metodiev, M., Fernández, N., Klapp, BF., Arck, PC. and Blois, SM., (2008). In vivo dendritic cell depletion reduces breeding efficiency, affecting implantation and early placental development in mice. Journal of Molecular Medicine. 86 (9), 999-1011

Shakhawat, A. and Fern�ndez, N., (2008). Effect of TNF alpha nd Interleukin 4 on HLA-G expression, cell proliferation and apoptosis of the human placental trophoblast Jeg-3 cell line. Immunology. 125, creators-Fern=E1ndez=3ANelson=3A=3A

Mavrakos, E., Morrison, IEG., Cherry, RJ. and Fern�ndez, N., (2008). Single molecule visualisation of the CD55, a decay accelerating factor, interaction with CD14 in the lipopolysaccharide signalling pathway. Immunology. 125, creators-Fern=E1ndez=3ANelson=3A=3A

Mavrakos, E., Kacharakkal, HJ., Yadav, N., O'Farrell, M. and Fern�ndez, N., (2008). The role of poly ADP-ribosylation in endotoxin-induced inflammatory processes in macrophages. Immunology. 125, creators-Fern=E1ndez=3ANelson=3A=3A

Garcia, MG., Tirado-Gonzalez, I., Handjiski, B., Tometten, M., Orsal, AS., Hajos, SE., Fernández, N., Arck, PC. and Blois, SM., (2007). High Expression of Survivin and Down-Regulation of Stat-3 Characterize the Feto-Maternal Interface in Failing Murine Pregnancies During the Implantation Period. Placenta. 28 (7), 650-657

Laskarin, G., Kämmerer, U., Rukavina, D., Thomson, AW., Fernandez, N. and Blois, SM., (2007). Antigen-Presenting Cells and Materno-Fetal Tolerance: An Emerging Role for Dendritic Cells. American Journal of Reproductive Immunology. 58 (3), 255-267

Cooper, JC., Dealtry, GB., Ahmed, MA., Arck, PC., Klapp, BF., Blois, SM. and Fernández, N., (2007). An Impaired Breeding Phenotype in Mice with a Genetic Deletion of Beta-2 Microglobulin and Diminished MHC Class I Expression: Role in Reproductive Fitness1. Biology of Reproduction. 77 (2), 274-279

Blois, SM., Kammerer, U., Soto, CA., Tometten, MC., Shaikly, V., Barrientos, G., Jurd, R., Rukavina, D., Thomson, AW., Klapp, BF., Fernández, N. and Arck, PC., (2007). Dendritic Cells: Key to Fetal Tolerance?1. Biology of Reproduction. 77 (4), 590-598

D'Arcy, V., Abdullaev, ZK., Pore, N., Docquier, F., Torrano, V., Chernukhin, I., Smart, M., Farrar, D., Metodiev, M., Fernandez, N., Richard, C., Delgado, MD., Lobanenkov, V. and Klenova, E., (2006). The Potential of BORIS Detected in the Leukocytes of Breast Cancer Patients as an Early Marker of Tumorigenesis. Clinical Cancer Research. 12 (20), 5978-5986

Morrison, IE., Karakikes, I., Barber, RE., Fernández, N. and Cherry, RJ., (2003). Detecting and Quantifying Colocalization of Cell Surface Molecules by Single Particle Fluorescence Imaging. Biophysical Journal. 85 (6), 4110-4121

Fernández, EM., O’Toole, PJ., Morrison, IE., Cherry, RJ. and Fernández, N., (2003). Interaction of HLA-DR with actin microfilaments. Human Immunology. 64 (3), 327-337

Cherry, R., Morrison, I., Karakikes, I., Barber, R., Silkstone, G. and Fernández, N., (2003). Measurements of associations of cell-surface receptors by single-particle fluorescence imaging. Biochemical Society Transactions. 31 (5), 1028-1031

Karakikes, I., Barber, R., Morrison, I., Fernández, N. and Cherry, R., (2003). Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging. Biochemical Society Transactions. 31 (6), 1453-1455

Sutton, JM., Clarke, OJ., Fernandez, N. and Boyle, RW., (2002). Porphyrin, Chlorin, and Bacteriochlorin Isothiocyanates:  Useful Reagents for the Synthesis of Photoactive Bioconjugates †. Bioconjugate Chemistry. 13 (2), 249-263

Georgiou, G., Bahra, SS., Mackie, AR., Wolfe, CA., O’Shea, P., Ladha, S., Fernandez, N. and Cherry, RJ., (2002). Measurement of the Lateral Diffusion of Human MHC Class I Molecules on HeLa Cells by Fluorescence Recovery after Photobleaching Using a Phycoerythrin Probe. Biophysical Journal. 82 (4), 1828-1834

Triantafilou, K., Triantafilou, M., Ladha, S., Mackie, A., Fernandez, N., Dedrick, RL. and Cherry, R., (2001). Erratum: Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane (Journal of Cell Science (2001) Vol. 114 (2535-2545)). Journal of Cell Science. 114 (17), 3072-

Triantafilou, K., Triantafilou, M., Ladha, S., Mackie, A., Fernandez, N., Dedrick, RL. and Cherry, R., (2001). Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane. Journal of Cell Science. 114 (13), 2535-2545

Triantafilou, K., Triantafilou, M., Ladha, S., Mackie, A., Dedrick, RL., Fernandez, N. and Cherry, R., (2001). Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane.. Journal of cell science. 114 (Pt 13), 2535-2545

Triantafilou, K., Triantafilou, M. and Fernandez, N., (2000). Molecular associations and microdomains in antigen-presenting cell-T-cell interactions. Critical Reviews in Immunology. 20 (5), 359-373

Triantafilou, K., Triantafilou, M., Takada, Y. and Fernandez, N., (2000). Human Parechovirus 1 Utilizes Integrins alpha vbeta 3 and alpha vbeta 1 as Receptors. Journal of Virology. 74 (13), 5856-5862

Bottley, G. and Fernandez, N., (2000). Dimerization of major histocompatibility complex class I on the surface of THP-1 cells stimulates the expression of inducible nitric oxide synthase and subsequent nitric oxide release. Immunology. 100 (4), 449-454

Dealtry, GB., O’Farrell, MK. and Fernandez, N., (2000). The Th2 Cytokine Environment of the Placenta. International Archives of Allergy and Immunology. 123 (2), 107-119

Triantafilou, M., Triantafilou, K. and Fernandez, N., (2000). Rough and smooth forms of fluorescein-labelled bacterial endotoxin exhibit CD14/LBP dependent and independent binding that is influencedby endotoxin concentration. European Journal of Biochemistry. 267 (8), 2218-2226

Triantafilou, M., Triantafilou, K., Wilson, KM., Takada, Y. and Fernandez, N., (2000). High affinity interactions of Coxsackievirus A9 with integrin αvβ3 (CD51/61) require the CYDMKTTC sequence of β3, but do not require the RGD sequence of the CAV-9 VP1 protein. Human Immunology. 61 (5), 453-459

Triantafilou, K., Triantafilou, M., Wilson, KM. and Fernandez, N., (2000). Human major histocompatibility molecules have the intrinsic ability to form homotypic associations. Human Immunology. 61 (6), 585-598

Triantafilou, K., Triantafilou, M. and Fernandez, N., (2000). Lipopolysaccharide (LPS) labeled with alexa 488 hydrazide as a novel probe for LPS binding studies. Cytometry. 41 (4), 316-320

Sutton, JM., Fernandez, N. and Boyle, RW., (2000). Functionalized diphenylchlorins and bacteriochlorins: Their synthesis and bioconjugation for targeted photodynamic therapy and tumour cell imaging. Journal of Porphyrins and Phthalocyanines. 4 (7), 655-658

Triantafilou, K., Triantafilou, M. and Fernandez, N., (2000). Lipopolysaccharide (LPS) labeled with Alexa 488 hydrazide as a novel probe for LPS binding studies. CYTOMETRY. 41 (4), 316-320

SUTTON, JM., FERNANDEZ, N. and BOYLE, RW., (2000). Functionalized diphenylchlorins and bacteriochlorins: their synthesis and bioconjugation for targeted photodynamic therapy and tumour cell imaging. Journal of Porphyrins and Phthalocyanines (JPP). 04 (07), 655-658

Fernandez, N., (1999). A critical review of the role of the major histocompatibility complex in fertilization, preimplantation development and feto-maternal interactions. Human Reproduction Update. 5 (3), 234-248

Fernandez, N., Triantafilou, K., Stanway, G., Wilson, KM., Triantafilou, M. and Takada, Y., (1999). Involvement of β2-microglobulin and integrin αvβ3 molecules in the coxsackievirus A9 infectious cycle. Journal of General Virology. 80 (10), 2591-2600

Triantafilou, K., Triantafilou, M., Wilson, KM., Cherry, RJ. and Fernandez, N., (1999). Intracellular and cell surface heterotypic associations of human leukocyte antigen-DR and human invariant chain. Human Immunology. 60 (11), 1101-1112

Smith, PR., Morrison, IE., Wilson, KM., Fernández, N. and Cherry, RJ., (1999). Anomalous Diffusion of Major Histocompatibility Complex Class I Molecules on HeLa Cells Determined by Single Particle Tracking. Biophysical Journal. 76 (6), 3331-3344

Nilsson, AM., Wijaywardene, M., Gkoutos, G., Wilson, KM., Fern�ndez, N. and Reynolds, CA., (1999). Correlated mutations in the HLA class II molecule. International Journal of Quantum Chemistry. 73 (2), 85-96

Triantafilou, M., Triantafilou, K., Wilson, KM., Takada, Y., Fernandez, N. and Stanway, G., (1999). Involvement of β2-microglobulin and integrin α(v)β3 molecules in the coxsackievirus A9 infectious cycle. Journal of General Virology. 80 (10), 2591-2600

COOPER, JC., FERNANDEZ, N., JOLY, E. and DEALTRY, GB., (1998). Regulation of Major Histocompatibility Complex and TAP Gene Products in Preimplantation Mouse Stage Embryos. American Journal of Reproductive Immunology. 40 (3), 165-171

Cherry, RJ., Wilson, KM., Triantafilou, K., O'Toole, P., Morrison, IE., Smith, PR. and Fernández, N., (1998). Detection of Dimers of Dimers of Human Leukocyte Antigen (HLA)–DR on the Surface of Living Cells by Single-Particle Fluorescence Imaging. The Journal of Cell Biology. 140 (1), 71-79

Cherry, RJ., Smith, PR., Morrison, IE. and Fernandez, N., (1998). Mobility of cell surface receptors: a re-evaluation. FEBS Letters. 430 (1-2), 88-91

Wilson, KM., Triantafilou, K., Morrison, IEG., Cherry, RJ. and Fernandez, N., (1997). SINGLE PARTICLE IMAGING OF CELL-SURFACE HLA-DR TETRAMERS. Biochemical Society Transactions. 25 (2), 360S-360S

Cherry, RJ., Smith, PR., Morrison, IEG., Koukidou, M., Wilson, KM. and Fernández, N., (1997). Mobility of cell-surface MHC molecules investigated by single-particle fluorescent imaging. Biochemical Society Transactions. 25 (3), 1097-1102

Triantafilou, K., Wilson, KM. and Fernandez, N., (1997). INTRACELLULAR AND CELL SURFACE MAPPING OF HLA DR IN THE PRESENCE AND ABSENCE OF THE INVARIANT CHAIN. Biochemical Society Transactions. 25 (2), 259S-259S

Triantafilou, K., Wilson, KM., Morrison, IEG., Cherry, RJ. and Fernandez, N., (1997). THE STATE OF AGGREGATION OF MHC CLASS II MOLECULES AT THE CELL-SURFACE IS TEMPERATURE DEPENDENT. Biochemical Society Transactions. 25 (2), 358S-358S

Fiszer, D., Ulbrecht, M., Fernandez, N., Johnson, JP., Weiss, EH. and Kurpisz, M., (1997). Analysis of HLA class Ib gene expression in male gametogenic cells. European Journal of Immunology. 27 (7), 1691-1695

Wilson, KM., Morrison, IEG., Smith, PR., Fernandez, N. and Cherry, RJ., (1996). Single particle tracking of cell-surface HLA-DR molecules using R-phycoerythrin labeled monoclonal antibodies and fluorescence digital imaging. Journal of Cell Science. 109 (8), 2101-2109

Janitz, M., Fiszer, D., Michalczak-Janitz, K., Lukaszyk, A., Fernandez, N., Skorupski, W. and Kurpisz, M., (1994). Analysis of mRNA for class I HLA on human gametogenic cells. Molecular Reproduction and Development. 38 (2), 231-237

Wilson, KM., Labeta, MO., Pawelec, G. and Fernandez, N., (1993). Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules. Immunology. 79 (2), 331-335

Sprinks, M., Sellens, M., Dealtry, G. and Fernandez, N., (1993). Preimplantation mouse embryos express Mhc class I genes before the first cleavage division. Immunogenetics. 38 (1), 35-40

Reyes-Engel, A., Le Roy, E., Diegues, JL. and Fernandez, N., (1993). Unusual expression pattern of the MHC class I Q5k gene in the AKR mouse: Possible role in embryo development. Journal of Reproductive Immunology. 23 (1), 73-81

Labeta, MO., Durieux, J-J., Fernandez, N., Herrmann, R. and Ferrara, P., (1993). Release from a human monocyte-like cell line of two different soluble forms of the lipopolysaccharide receptor, CD14. European Journal of Immunology. 23 (9), 2144-2151

Labeta, MO., Durieux, JJ., Spagnoli, G., Fernandez, N., Wijdenes, J. and Herrmann, R., (1993). CD14 and tolerance to lipopolysaccharide: Biochemical and functional analysis. Immunology. 80 (3), 415-423

Fernandez, N., Kurpisz, M., Labeta, M., Sachs, J. and Pawelec, G., (1992). A ligand-epitope in vitro analysis of major histocompatibility determinants expressed on B and T lymphocytes. Immunology. 77 (1), 116-122

Fiszer, D., Niedbala, W., Fernandez, N. and Kurpisz, M., (1992). The cytokines effect of EBV-immortalized human B cells producing antisperm antibody. Archivum Immunologiae et Therapiae Experimentalis. 40 (3-4), 247-251

KURPISZ, M. and FERNANDEZ, N., (1992). Main Histocompatibility Complex and Reproductive System. American Journal of Reproductive Immunology. 28 (1), 19-30

Fernandez, N. and Klidis, A., (1991). Molecular profiles of the cell membrane bound and cytoplasmic forms of the human MHC Class-II associated invariant polypeptides. Electrophoresis. 12 (7-8), 523-526

Delgado, V., Ramos, T., Delgado, E., Fernandez, N., Diez, ML., Romera, N. and Mateos, F., (1990). Effect of hepatic ischemic reperfusion on the adenine nucleotides: Its tissue degradation and integrity. Alopurinol-protecting effect. Revista de Diagnostico Biologico. 39 (3), 127-135

Fernandez, N., Labeta, M., Kurpisz, M., Nakatsuji, T., Sachs, J. and Festenstein, H., (1990). Major histocompatibility complex (MHC) protein analysis by optimised two-dimensional electrophoretic methods. Electrophoresis. 11 (2), 167-174

Labeta, M., Fernandez, N. and Festenstein, H., (1989). The expression of an 'alloantigen'-like molecule is a general phenomenon in AKR leukaemias. Transplantation Proceedings. 21 (1 I), 578-

Labeta, MO., Fernandez, N., Reyes, A., Ferrara, P., Marelli, O., Le Roy, E., Houlihan, J. and Festenstein, H., (1989). Biochemical analysis of a novel H-2 class I-like glycoprotein expressed in five AKR-(Gross virus) derived spontaneous T cell leukemias. Journal of Immunology. 143 (4), 1245-1253

Pawelec, G., Brocker, T., Busch, FW., Buhring, HJ., Fernandez, N., Schneider, EM. and Wernet, P., (1988). 'Tolerization' of human T-helper cell clones by chronic exposure to alloantigen: Culture conditions dictate autocrine proliferative status but not acquisition of cytotoxic potential and suppressor-induction capacity. Journal of Molecular and Cellular Immunology. 4 (1), 21-34

Fernandez, N., Hitman, GA., Festenstein, H., Garde, L., Labeta, M., Walker-Smith, JA. and Sachs, JA., (1988). Novel HLA class II-associated structural patterns in coeliac disease and type I diabetes. Clinical and Experimental Immunology. 72 (3), 362-366

Fernandez, N., Labeta, MO. and Festenstein, H., (1988). A novel HLA class II molecule. Immunogenetics. 27 (5), 363-369

Pawelec, G., Fernandez, N., Kalthoff, F., Buhring, HJ., Busch, FW., Schneider, EM., Festenstein, H. and Wernet, P., (1988). Induction of 'tolerance' in monoclonal human helper T lymphocyte populations during culture under conditions of chronic alloantigenic stimulation. Transplantation Proceedings. 20 (2), 164-166

Pawelec, G., (1988). DY determinants, possibly associated with novel class II molecules, stimulate autoreactive CD4+ T cells with suppressive activity. Journal of Experimental Medicine. 167 (2), 243-261

Labeta, MO., Fernandez, N. and Festenstein, H., (1988). Solubilisation effect of Nonidet P-40, Triton X-100 and CHAPS in the detection of MHC-like glycoproteins. Journal of Immunological Methods. 112 (1), 133-138

Labeta, MO., Fernandez, N. and Festenstein, H., (1987). Analytical studies of the binding parameters describing the interaction of HLA-DR epitopes with a specific monomorphic monoclonal antibody. Journal of Immunological Methods. 101 (1), 15-21

Kurpisz, M., Fernandez, N., Witt, M., Kowalik, I., Szymczynski, GA. and Festenstein, H., (1987). HLA EXPRESSION ON HUMAN GERMINAL CELLS. European Journal of Immunogenetics. 14 (1), 23-32

Fernandez, N., Labeta, M., Kurpisz, M., Solana, R. and Festenstein, H., (1986). HLA-DQ molecular heterogeneity in HLA-DR4-Dw4 consanguineous cell lines. Disease Markers. 4 (1-2), 191-198

Fernandez, N., Festenstein, H., Alonzo, A., Biro, PA., Labeta, M., Carolan, E., Houlihan, J., Mellor, A. and Flavell, R., (1986). THE EXPRESSION OF MURINE Qa REGION GENE PRODUCT(S) IN L CELL TRANSFORMANTS. European Journal of Immunogenetics. 13 (5-6), 425-436

Navarrete, C., Fernandez, N., Alonso, M. and Festenstein, H., (1986). Ontogenic and functional implications of the differential expression of HLA-DQ antigens on leukemic cells. Human Immunology. 16 (1), 52-68

Sachs, JA., Fernandez, N., Kurpisz, M., Okoye, R., Ogilvie, J., Awad, J., Labeta, M. and Festenstein, H., (1986). Serological biochemical and functional characterisation of three different HLA-DR monoclonal antibodies derived from C57BL6 mice. Tissue Antigens. 28 (4), 199-207

Kurpisz, M., Fernandez, N., Szymczyński, G. and Kowalik, I., (1986). The non-mature sperm cells: evaluation of surface markers and interaction in in vitro cultures.. Archivum immunologiae et therapiae experimentalis. 34 (1), 101-109

KURPISZ, M., MAZURKIEWICZ, I. and FERNANDEZ, N., (1985). Morphological and Immunological Observations in Experimentally Induced Torsion of Testis in Rats. American Journal of Reproductive Immunology and Microbiology. 9 (4), 129-135

Fernandez, N., Solana, R. and Sachs, JA., (1984). Characterization of 59 workshop monoclonal antibodies using panels of EBV transformed HTCs and deletion mutant cell lines. Disease Markers. 2 (1-2), 197-205

Solana, R., Fernandez, N. and Alonso, MC., (1984). Comparison of RIA and ELISA for screening class II monoclonal antibodies with EBV transformed cell lines. Disease Markers. 2 (1-2), 33-37

Conferences (35)

Muhandiram, N., Jabeen, A. and Fernàndez, N., (2015). Bacterial LPS differentially modulates the expression of Myoferlin in trophoblast cells

Alssadh, H., Alabdulmenaim, W. and Fernandez, N., (2014). Analysis of the expression and interrelationship between the CD74 and CD44 receptors expressed on human breast cancer derived cells

Alabdulmenaim, W., Al Ssadh, H. and Fernandez, N., (2014). Lack of Trophoblast-Specific Isoforms of CD74 May Protect Pregnancy

Al Ssadh, H., Fernandez, N. and Alabdulmenaim, W., (2014). Analysis of the Expression and Interrelationship Between the CD74 and CD44 Receptors Expressed on Human Breast Cancer Derived Cells

Hakam, SM., Pallavi, JH., Sachiyo, T., Barnea, ER. and Fernandez, N., (2014). Preimplantation factor (PIF*) regulates HLA-G expression at the feto-maternal interface

Jain, P., Blanch, A., Jabeen, A., Hakam, SM., Laissue, P. and Fernandez, N., (2012). THE INTERACTION OF THE CYTOSKELETON WITH HISTOCOMPATIBILITY MOLECULES ON TROPHOBLAST CELLS: RELEVANCE FOR FOETO-MATERNAL TOLERANCE

Jain, P., Blanch, A., Jabeen, A., Hakam, S., Laissue, P. and Fernandez, N., (2012). The effect of all-trans retinoic acid on histocompatibility receptor distribution at the foeto-maternal interface

McElhinney, JMWR., Sayago, JMM., Taylor, OW., Reynolds, CA. and Fernandez, N., (2012). Serum albumin modulates pro-inflammatory responses induced by bacterial lipopolysaccharides in macrophage-like MM6 cells

Jabeen, A., Laissue, PP., Obara, B., Jain, P. and Fernandez, N., (2012). Immunological receptors' expression and communication on extravillous trophoblast cell surface

Blanch, A., Jain, P., Jabeen, A. and Fernandez, N., (2012). The role of the cytoskeleton in foeto-maternal immunotolerance

Jabeen, A., Alkhatib, S., Hicks, C., Uddin, T., Hakam, S., Jain, P. and Fernández, N., (2012). Extra-villous trophoblast cytoskeleton deformation under co-infection with viral and bacterial agents

Lee, C-L., Lam, KK., Koistinen, H., Seppala, M., Kurpisz, M., Fernandez, N., Pang, RT., Yeung, WS. and Chiu, PC., (2011). Glycodelin-A as a paracrine regulator in early pregnancy

Jabeen, A., Laissue, PP., Jain, P., Shakhawat, A., Shaikly, V. and Fernandez, N., (2011). Study of MHC class I molecules associations: Is receptor interaction at the cell-surface important at the feto-maternal interface?

Jabeen, A., Jain, P., Laissue, PP., Suhling, K., Levitt, JA., Shaikly, V., Shakhawat, A. and Fernandez, N., (2010). Physical interactions of HLA-G with MHC class I partner molecules on the surface of two trophoblast cell lines, JEG-3 and ACH-3P, by Förster resonance energy transfer based on fluorescence lifetime measurements

Shakhawat, A., Shaikly, V. and Fernández, N., (2010). Anti-inflammatory interleukin-4 (IL-4) cytokine regulates human leukocyte antigen-G expression in a trophoblast cell line

Shaikly, V., Shakhawat, A., Morrison, I., Mavrakos, E., Cherry, AJR. and Fernández, N., (2009). MHC profiles in human pre-implantation embryos

Shakhawat, A., Shaikly, V., Mavrakos, E. and Fernández, N., (2009). The protective roles of non-classical MHC class Ib HLA-G antigen against maternal macrophages

Mavrakos, E., Kacharakkal, HJ., Yadav, N., O'Farrell, M. and Fernandez, N., (2008). The role of poly ADP-ribosylation in endotoxin-induced inflammatory processes in macrophages

Mavrakos, E., Morrison, IG., Cherry, RJ. and Fernandez, N., (2008). Single molecule visualisation of the CD55, a decay accelerating factor, interaction with CD14 in the lipopolysaccharide signalling pathway

Shakhawat, A. and Fernandez, N., (2008). Effect of TNF alpha nd Interleukin 4 on HLA-G expression, cell proliferation and apoptosis of the human placental trophoblast Jeg-3 cell line

Shaikly, V., Taranissi, M., Seaton, A. and Fernandez, N., (2006). Analysis of soluble HLA-G in the culture media of human preimplantation embryos from cleavage stages to blastocyst and embryos undergoing preimplantation genetic diagnosis

Nebenfuhr, MT., Morrison, IEG., Karakikes, I., Cherry, RJ. and Fernandez, N., (2005). Detection and quantification of colocalization of CD14 and TLR4 upon LPS stimulation on human monocytes using single particle fluorescence imaging

Cherry, RJ., Morrison, IEG., Karakikes, I., Barber, RE., Silkstone, G. and Fernández, N., (2003). Measurements of associations of cell-surface receptors by single-particle fluorescence imaging

Karakikes, I., Barber, RE., Morrison, IEG., Fernández, N. and Cherry, RJ., (2003). Co-localization of cell surface receptors at high spatial resolution by single-particle fluorescence imaging

WEISS, EH., CANNICH, A., SPRINKS, M., FERNANDEZ, N. and ULBRECHT, M., (1998). Unique Biochemical Properties of Human Leukocyte Antigen-E Allow for a Highly Specific Function in Immune Recognition

Wilson, KM., Morrison, IEG., Cherry, RJ. and Fernandez, N., (1997). In vivo single particle imaging reveals the formation of cell surface HLA-DR dimer of dimers

Fernandez, N. and Kurpisz, M., (1996). The major histocompatibility complex and the developing mammalian embryo

Triantafilou, K., Wilson, KM., Morrison, IEG., Cherry, RJ. and Fernandez, N., (1996). The state of aggregation of MHC Class II molecules at the cell-surface is temperature dependent

Wilson, KM., Triantafilou, K., Morrison, IEG., Cherry, RJ. and Fernandez, N., (1996). Single particle imaging of cell-surface HLA-DR tetramers

Triantafilou, K., Wilson, KM. and Fernandez, N., (1996). Intracellular and cell surface mapping of HLA DR in the presence and absence of the invariant chain

Wilson, KM., Smith, PR., Morrison, IEG., Cherry, RJ. and Fernandez, N., (1996). The mobility and self-associations of MHC receptors at the cell surface of antigen presenting cells

Cooper, JC., Fernandez, N. and Dealtry, G., (1996). Ontogeny and role of the MHC in mouse embryonic development

Fernandez, N., Cooper, JC., Sprinks, MT. and Dealtry, GB., (1996). Impaired reproduction of the B-2-M deficient transgenic

Sprinks, MT., Ulbrecht, M., Fernandez, N. and Weiss, EH., (1996). Identification of the human non-classical MHC class I HLA-E molecule by 1-D IEF

Smith, PR., Wilson, KM., Morrison, IEG., Fernandez, N. and Cherry, RJ., (1996). Comparitive analysis of MHC class I and class II cell surface organisation using digital fluorescence imaging

Grants and funding

2016

The role of soluble factors in regulating maternally derived fibroblasts

Wellcome Trust

2015

Is fetomaternal tolerance linked to HLA expression on trophoblasts

Wellcome Trust

2014

Biomedical Vacation Scholarships - 2014 (Mr Akira O Williamson)

Wellcome Trust

Contact

nelson@essex.ac.uk
+44 (0) 1206 873330

Location:

3SW.4.22, Colchester Campus