People

Dr Richard Strange

Senior Research Fellow
School of Life Sciences
Dr Richard Strange
  • Email

  • Location

    Colchester Campus

  • Academic support hours

    Final Year Project students (3) Protein Bioinformatics (2 lectures, 3 Workshops) MSc research Project student PhD student

Profile

Biography

1992-2008 Daresbury Synchrotron Radiation Laboratory 2008-2015 Senior Lecturer, University of Liverpool Fellow, Institute of Physics Senior Associate, Royal Society of Medicine Visiting Scientist, RIKEN, SPring-8 (Harima), Japan Co-editor, Journal of Synchrotron Radiation Editorial Board member, Nature Scientific Reports

Qualifications

  • BSc(Hons) Physics & Philosophy

  • PhD Physics

Research and professional activities

Research interests

The overall theme of my research is based on reaching an understanding of structure-function relationship of proteins, enzymes and their complexes, including those involved in disease (e.g. ALS), via advanced computational and experimental approaches. This encompasses synchrotron radiation, protein crystallography, XAFS, SAXS, SAD phasing, catalysis, structure-based drug discovery, molecular dynamics and computational chemistry.

Current research

Dynamics of Electron and Proton Transfer Chemistry in Copper and Hybrid Copper-Haem Enzymes

Multiple Structures from One Crystal (MSOX) enzyme catalysis and variable temperature crystallography, allied to molecular dynamics and QM/MM simulations

Conferences and presentations

Chair

Q2XAFS2017, Q2XAFS2017, Didcot, United Kingdom, 14/8/2017

Solvent Accessibility and Ligand Binding in Native Achromobacter cycloclastes Copper Nitrite Reductase

19th IUPAB congress and 11th EBSA congress, 19th IUPAB congress and 11th EBSA congress, Edinburgh, United Kingdom, 7/2017

The T2Cu active site in copper containing nitrite reductases: water accessibility and nitrite binding

Second CCPBioSim/CCP5 Multiscale Modelling Conference, Second CCPBioSim/CCP5 Multiscale Modelling Conference, Manchester, United Kingdom, 4/2016

British Biophysical Society conference on 'New Horizons and Emerging Biomedical Challenges for Biophysics', Liverpool July 2016

Liverpool, United Kingdom, 2016

23rd IUCR Congress, Montreal, Canada August 2014. Organiser and Chair, microsymposium on Electronic Structure and Chemical Bond Information by High Energy Resolution X-ray Spectroscopy

Montreal, Canada, 2014

38th International Conference on Vacuum Ultraviolet and X-ray Physics, Hefei, China July 2013

Hefei, China, 2013

Seminar series, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, November 2012

Nanyang, China, 2012

10th International Consortium on SOD1 and ALS, Liverpool March 2012

Liverpool, United Kingdom, 2012

Teaching and supervision

Previous supervision

Silvia Atanasio
Silvia Atanasio
Thesis title: Insight Into G Protein-Coupled Receptors Through Molecular Dynamics Simulations
Degree subject: Biological Sciences
Degree type: Doctor of Philosophy
Awarded date: 26/1/2021
Tadeo Moreno Chicano
Tadeo Moreno Chicano
Thesis title: Structural Characterisation of Ligand, Redox and Catalytic States in Haem Enzymes Using in Crystallo Spectroscopies and Serial Crystallography.
Degree subject: Biological Sciences
Degree type: Doctor of Philosophy
Awarded date: 23/4/2019

Publications

Journal articles (114)

Hough, MA., Conradie, J., Strange, RW., Antonyuk, SV., Eady, RR., Ghosh, A. and Hasnain, SS., (2020). Nature of the copper-nitrosyl intermediates of copper nitrite reductases during catalysis. Chemical Science. 11 (46), 12485-12492

Ebrahim, A., Appleby, MV., Axford, D., Beale, J., Moreno-Chicano, T., Sherrell, DA., Strange, RW., Hough, MA. and Owen, RL., (2019). Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography. Acta Crystallographica Section D Structural Biology. 75 (2), 151-159

Moreno-Chicano, T., Ebrahim, A., Axford, D., Appleby, MV., Beale, JH., Chaplin, AK., Duyvesteyn, HME., Ghiladi, RA., Owada, S., Sherrell, DA., Strange, RW., Sugimoto, H., Tono, K., Worrall, JAR., Owen, RL. and Hough, MA., (2019). High-throughput structures of protein–ligand complexes at room temperature using serial femtosecond crystallography. IUCrJ. 6 (6), 1074-1085

Ebrahim, A., Moreno-Chicano, T., Appleby, MV., Chaplin, AK., Beale, JH., Sherrell, DA., Duyvesteyn, HME., Owada, S., Tono, K., Sugimoto, H., Strange, RW., Worrall, JAR., Axford, D., Owen, RL. and Hough, MA., (2019). Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins. IUCrJ. 6 (4), 543-551

Manjula, R., Wright, GSA., Strange, RW. and Padmanabhan, B., (2018). Assessment of ligand binding at a site relevant to SOD 1 oxidation and aggregation. FEBS Letters. 592 (10), 1725-1737

Horrell, S., Kekilli, D., Sen, K., Owen, RU., Dworkowski, FSN., Antonyuk, SV., Keal, TW., Yong, CW., Eady, RR., Hasnain, SS., Strange, RW. and Hough, MA., (2018). Enzyme catalysis captured using multiple structures from one crystal at varying temperatures. IUCrJ. 5 (3), 283-292

Diaz-Moreno, S. and Strange, RW., (2018). Third International Workshop on Improving Data Quality in XAFS Spectroscopy, Q2XAFS2017. Journal of Synchrotron Radiation. 25 (4), 918-919

Bielecki, M., Antonyuk, S., Strange, RW., Smalley, JW., Mackiewicz, P., Śmiga, M., Stępień, P., Olczak, M. and Olczak, T., (2018). Tannerella forsythia Tfo belongs to Porphyromonas gingivalis HmuY-like family of proteins but differs in heme-binding properties. Bioscience Reports. 38 (5)

Sen, K., Hough, M., Strange, R., Yong, C. and Keal, T., (2018). A QM/MM Study of Nitrite Binding Modes in a Three-Domain Heme-Cu Nitrite Reductase. Molecules. 23 (11), 2997-2997

Bellini, D., Horrell, S., Hutchin, A., Phippen, CW., Strange, RW., Cai, Y., Wagner, A., Webb, JS., Tews, I. and Walsh, MA., (2017). Dimerisation induced formation of the active site and the identification of three metal sites in EAL-phosphodiesterases. Scientific Reports. 7 (1), 42166-

Kekilli, D., Petersen, CA., Pixton, DA., Ghafoor, DD., Abdullah, GH., Dworkowski, FSN., Wilson, MT., Heyes, DJ., Hardman, SJO., Murphy, LM., Strange, RW., Scrutton, NS., Andrew, CR. and Hough, MA., (2017). Engineering proximal vs. distal heme–NO coordination via dinitrosyl dynamics: implications for NO sensor design. Chemical Science. 8 (3), 1986-1994

Kekilli, D., Moreno-Chicano, T., Chaplin, AK., Horrell, S., Dworkowski, FSN., Worrall, JAR., Strange, RW. and Hough, MA., (2017). Photoreduction and validation of haem?ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction. IUCrJ. 4 (3), 263-270

Sen, K., Horrell, S., Kekilli, D., Yong, CW., Keal, TW., Atakisi, H., Moreau, DW., Thorne, RE., Hough, MA. and Strange, RW., (2017). Active-site protein dynamics and solvent accessibility in nativeAchromobacter cycloclastescopper nitrite reductase. IUCrJ. 4 (4), 495-505

Horrell, S., Kekilli, D., Strange, RW. and Hough, MA., (2017). Recent structural insights into the function of copper nitrite reductases.. Metallomics. 9 (11), 1470-1482

Nilsson, ZN., Mandella, BL., Sen, K., Kekilli, D., Hough, MA., Moënne-Loccoz, P., Strange, RW. and Andrew, CR., (2017). Distinguishing Nitro vs Nitrito Coordination in Cytochrome c' Using Vibrational Spectroscopy and Density Functional Theory.. Inorganic Chemistry. 56 (21), 13205-13213

Horrell, S., Antonyuk, SV., Eady, RR., Hasnain, SS., Hough, MA. and Strange, RW., (2016). Serial crystallography captures enzyme catalysis in copper nitrite reductase at atomic resolution from one crystal. IUCrJ. 3 (4), 271-281

Ghafoor, DD., Kekilli, D., Abdullah, GH., Dworkowski, FSN., Hassan, HG., Wilson, MT., Strange, RW. and Hough, MA., (2015). Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c’. JBIC Journal of Biological Inorganic Chemistry. 20 (6), 949-956

Capper, MJ., O?Neill, PM., Fisher, N., Strange, RW., Moss, D., Ward, SA., Berry, NG., Lawrenson, AS., Hasnain, SS., Biagini, GA. and Antonyuk, SV., (2015). Antimalarial 4(1H)-pyridones bind to the Qisite of cytochromebc1. Proceedings of the National Academy of Sciences. 112 (3), 755-760

Kekilli, D., Dworkowski, FSN., Pompidor, G., Fuchs, MR., Andrew, CR., Antonyuk, S., Strange, RW., Eady, RR., Hasnain, SS. and Hough, MA., (2014). Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy. Acta Crystallographica Section D Biological Crystallography. 70 (5), 1289-1296

Chng, C. and Strange, RW., (2014). Lipid-associated aggregate formation of superoxide dismutase-1 is initiated by membrane-targeting loops. Proteins: Structure, Function, and Bioinformatics. 82 (11), 3194-3209

Antonyuk, SV., Olczak, M., Olczak, T., Ciuraszkiewicz, J. and Strange, RW., (2014). The structure of a purple acid phosphatase involved in plant growth and pathogen defence exhibits a novel immunoglobulin-like fold. IUCrJ. 1 (2), 101-109

Kershaw, NM., Wright, GSA., Sharma, R., Antonyuk, SV., Strange, RW., Berry, NG., O'Neill, PM. and Hasnain, SS., (2013). X-ray Crystallography and Computational Docking for the Detection and Development of Protein–Ligand Interactions. Current Medicinal Chemistry. 20 (4), 569-575

Wright, GSA., Antonyuk, SV., Kershaw, NM., Strange, RW. and Samar Hasnain, S., (2013). Ligand binding and aggregation of pathogenic SOD1. Nature Communications. 4 (1), 1758-

Padmanabhan, B., Nakamura, Y., Antonyuk, SV., Strange, RW., Hasnain, SS., Yokoyama, S. and Bessho, Y., (2013). Structure of the hypothetical DUF1811-family protein GK0453 fromGeobacillus kaustophilusHTA426. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 69 (4), 342-345

Wright, GSA., Lee, HC., Schulze-Briese, C., Grossmann, JG., Strange, RW. and Hasnain, SS., (2013). The application of hybrid pixel detectors for in-house SAXS instrumentation with a view to combined chromatographic operation.. Journal of Synchrotron Radiation. 20 (Pt 2), 383-385

Strange, RW., Hough, MA., Antonyuk, SV. and Hasnain, SS., (2012). Structural Evidence for a Copper-Bound Carbonate Intermediate in the Peroxidase and Dismutase Activities of Superoxide Dismutase. PLoS ONE. 7 (9), e44811-e44811

Doutch, J., Hough, MA., Hasnain, SS. and Strange, RW., (2012). Challenges of sulfur SAD phasing as a routine method in macromolecular crystallography. Journal of Synchrotron Radiation. 19 (1), 19-29

Antonyuk, S., Strange, RW. and Hasnain, SS., (2010). Structural Discovery of Small Molecule Binding Sites in Cu−Zn Human Superoxide Dismutase Familial Amyotrophic Lateral Sclerosis Mutants Provides Insights for Lead Optimization. Journal of Medicinal Chemistry. 53 (3), 1402-1406

Galaleldeen, A., Strange, RW., Whitson, LJ., Antonyuk, SV., Narayana, N., Taylor, AB., Schuermann, JP., Holloway, SP., Hasnain, SS. and Hart, PJ., (2009). Structural and biophysical properties of metal-free pathogenic SOD1 mutants A4V and G93A. Archives of Biochemistry and Biophysics. 492 (1-2), 40-47

Yong, CW., Glab, J., Strange, RW., Smith, W., Hasnain, SS. and Grossmann, JG., (2009). Assessment of long-term molecular dynamics calculations with experimental information on protein shape from X-ray scattering – SOD1 as a case study. Chemical Physics Letters. 481 (1-3), 112-117

Hearnshaw, S., West, C., Singleton, C., Zhou, L., Kihlken, MA., Strange, RW., Le Brun, NE. and Hemmings, AM., (2009). A Tetranuclear Cu(I) Cluster in the Metallochaperone Protein CopZ. Biochemistry. 48 (40), 9324-9326

Antonyuk, SV., Strange, RW., Marklund, SL. and Hasnain, SS., (2009). The Structure of Human Extracellular Copper–Zinc Superoxide Dismutase at 1.7 Å Resolution: Insights into Heparin and Collagen Binding. Journal of Molecular Biology. 388 (2), 310-326

Antonyuk, SV., Trevitt, CR., Strange, RW., Jackson, GS., Sangar, D., Batchelor, M., Cooper, S., Fraser, C., Jones, S., Georgiou, T., Khalili-Shirazi, A., Clarke, AR., Hasnain, SS. and Collinge, J., (2009). Crystal structure of human prion protein bound to a therapeutic antibody. Proceedings of the National Academy of Sciences. 106 (8), 2554-2558

Padmanabhan, B., Bessho, Y., Ebihara, A., Antonyuk, SV., Ellis, MJ., Strange, RW., Kuramitsu, S., Watanabe, N., Hasnain, SS. and Yokoyama, S., (2009). Structure of putative 4-amino-4-deoxychorismate lyase fromThermus thermophilusHB8. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1234-1239

Antonyuk, SV., Strange, RW., Ellis, MJ., Bessho, Y., Kuramitsu, S., Shinkai, A., Yokoyama, S. and Hasnain, SS., (2009). Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) fromSulfolobus tokodaii. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1200-1203

Strange, RW., Antonyuk, SV., Ellis, MJ., Bessho, Y., Kuramitsu, S., Shinkai, A., Yokoyama, S. and Hasnain, SS., (2009). Structure of a putative β-phosphoglucomutase (TM1254) fromThermotoga maritima. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1218-1221

Antonyuk, SV., Ellis, MJ., Strange, RW., Bessho, Y., Kuramitsu, S., Shinkai, A., Yokoyama, S. and Hasnain, SS., (2009). Structure of SurE protein fromAquifex aeolicusVF5 at 1.5 Å resolution. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1204-1208

Padmanabhan, B., Strange, RW., Antonyuk, SV., Ellis, MJ., Hasnain, SS., Iino, H., Agari, Y., Bessho, Y. and Yokoyama, S., (2009). Structure of dihydrodipicolinate synthase fromMethanocaldococcus jannaschii. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1222-1226

Malay, AD., Bessho, Y., Ellis, MJ., Antonyuk, SV., Strange, RW., Hasnain, SS., Shinkai, A., Padmanabhan, B. and Yokoyama, S., (2009). Structure of glyceraldehyde-3-phosphate dehydrogenase from the archaeal hyperthermophileMethanocaldococcus jannaschii. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1227-1233

Strange, RW., Antonyuk, SV., Ellis, MJ., Bessho, Y., Kuramitsu, S., Yokoyama, S. and Hasnain, SS., (2009). The structure of an archaeal ribose-5-phosphate isomerase fromMethanocaldococcus jannaschii(MJ1603). Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1214-1217

Antonyuk, SV., Strange, RW., Ellis, MJ., Bessho, Y., Kuramitsu, S., Inoue, Y., Yokoyama, S. and Hasnain, SS., (2009). Structure ofD-lactate dehydrogenase fromAquifex aeolicuscomplexed with NAD+and lactic acid (or pyruvate). Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65 (12), 1209-1213

Ascone, I. and Strange, R., (2009). Biological X-ray absorption spectroscopy and metalloproteomics. Journal of Synchrotron Radiation. 16 (3), 413-421

Hough, MA., Antonyuk, SV., Strange, RW., Eady, RR. and Hasnain, SS., (2008). Crystallography with Online Optical and X-ray Absorption Spectroscopies Demonstrates an Ordered Mechanism in Copper Nitrite Reductase. Journal of Molecular Biology. 378 (2), 353-361

Strange, RW. and Feiters, MC., (2008). Biological X-ray absorption spectroscopy (BioXAS): a valuable tool for the study of trace elements in the life sciences. Current Opinion in Structural Biology. 18 (5), 609-616

Cao, X., Antonyuk, SV., Seetharaman, SV., Whitson, LJ., Taylor, AB., Holloway, SP., Strange, RW., Doucette, PA., Valentine, JS., Tiwari, A., Hayward, LJ., Padua, S., Cohlberg, JA., Hasnain, SS. and Hart, PJ., (2008). Structures of the G85R Variant of SOD1 in Familial Amyotrophic Lateral Sclerosis. Journal of Biological Chemistry. 283 (23), 16169-16177

Strange, RW., Yong, CW., Smith, W. and Hasnain, SS., (2007). Molecular dynamics using atomic-resolution structure reveal structural fluctuations that may lead to polymerization of human Cu Zn superoxide dismutase. Proceedings of the National Academy of Sciences. 104 (24), 10040-10044

Arcovito, A., Benfatto, M., Cianci, M., Hasnain, SS., Nienhaus, K., Nienhaus, GU., Savino, C., Strange, RW., Vallone, B. and Della Longa, S., (2007). X-ray structure analysis of a metalloprotein with enhanced active-site resolution using in situ x-ray absorption near edge structure spectroscopy. Proceedings of the National Academy of Sciences. 104 (15), 6211-6216

Rosenblum, G., Van den Steen, PE., Cohen, SR., Grossmann, JG., Frenkel, J., Sertchook, R., Slack, N., Strange, RW., Opdenakker, G. and Sagi, I., (2007). Insights into the Structure and Domain Flexibility of Full-Length Pro-Matrix Metalloproteinase-9/Gelatinase B. Structure. 15 (10), 1227-1236

Barrett, ML., Harvey, I., Sundararajan, M., Surendran, R., Hall, JF., Ellis, MJ., Hough, MA., Strange, RW., Hillier, IH. and Hasnain, SS., (2006). Atomic Resolution Crystal Structures, EXAFS, and Quantum Chemical Studies of Rusticyanin and Its Two Mutants Provide Insight into Its Unusual Properties†,‡. Biochemistry. 45 (9), 2927-2939

Strange, RW., Antonyuk, SV., Hough, MA., Doucette, PA., Valentine, JS. and Hasnain, SS., (2006). Variable Metallation of Human Superoxide Dismutase: Atomic Resolution Crystal Structures of Cu–Zn, Zn–Zn and As-isolated Wild-type Enzymes. Journal of Molecular Biology. 356 (5), 1152-1162

Yong, CW., Smith, W., Strange, RW. and Hasnain, SS., (2006). Structural investigation of human wild-type Cu, Zn superoxide dismutase – a biomolecular case study using DL_POLY_3. Molecular Simulation. 32 (12-13), 963-969

Antonyuk, S., Elam, JS., Hough, MA., Strange, RW., Doucette, PA., Rodriguez, JA., Hayward, LJ., Valentine, JS., Hart, PJ. and Hasnain, SS., (2005). Structural consequences of the familial amyotrophic lateral sclerosis SOD1 mutant His46Arg. Protein Science. 14 (5), 1201-1213

Hough, MA., Ellis, MJ., Antonyuk, S., Strange, RW., Sawers, G., Eady, RR. and Samar Hasnain, S., (2005). High Resolution Structural Studies of Mutants Provide Insights into Catalysis and Electron Transfer Processes in Copper Nitrite Reductase. Journal of Molecular Biology. 350 (2), 300-309

Strange, RW., Ellis, M. and Hasnain, SS., (2005). Atomic resolution crystallography and XAFS. Coordination Chemistry Reviews. 249 (1-2), 197-208

Antonyuk, SV., Strange, RW., Sawers, G., Eady, RR. and Hasnain, SS., (2005). Atomic resolution structures of resting-state, substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism. Proceedings of the National Academy of Sciences. 102 (34), 12041-12046

Hough, MA., Grossmann, JG., Antonyuk, SV., Strange, RW., Doucette, PA., Rodriguez, JA., Whitson, LJ., Hart, PJ., Hayward, LJ., Valentine, JS. and Hasnain, SS., (2004). Dimer destabilization in superoxide dismutase may result in disease-causing properties: Structures of motor neuron disease mutants. Proceedings of the National Academy of Sciences. 101 (16), 5976-5981

Ellis, MJ., Antonyuk, SV., Strange, RW., Sawers, G., Eady, RR. and Hasnain, SS., (2004). Observation of an Unprecedented Cu Bis-His Site: Crystal Structure of the H129V Mutant of Nitrite Reductase. Inorganic Chemistry. 43 (24), 7591-7593

Strange, RW., Antonyuk, S., Hough, MA., Doucette, PA., Rodriguez, JA., Hart, PJ., Hayward, LJ., Valentine, JS. and Hasnain, SS., (2003). The Structure of Holo and Metal-deficient Wild-type Human Cu, Zn Superoxide Dismutase and its Relevance to Familial Amyotrophic Lateral Sclerosis. Journal of Molecular Biology. 328 (4), 877-891

Strange, RW., Eady, RR., Lawson, D. and Hasnain, SS., (2003). XAFS studies of nitrogenase: the MoFe and VFe proteins and the use of crystallographic coordinates in three-dimensional EXAFS data analysis. Journal of Synchrotron Radiation. 10 (1), 71-75

Hasnain, SS. and Strange, RW., (2003). Marriage of XAFS and crystallography for structure–function studies of metalloproteins. Journal of Synchrotron Radiation. 10 (1), 9-15

Elam, JS., Taylor, AB., Strange, R., Antonyuk, S., Doucette, PA., Rodriguez, JA., Hasnain, SS., Hayward, LJ., Valentine, JS., Yeates, TO. and Hart, PJ., (2003). Amyloid-like filaments and water-filled nanotubes formed by SOD1 mutant proteins linked to familial ALS. Nature Structural & Molecular Biology. 10 (6), 461-467

Antonyuk, SV., Eady, RR., Strange, RW. and Hasnain, SS., (2003). The structure of glyceraldehyde 3-phosphate dehydrogenase fromAlcaligenes xylosoxidansat 1.7 Å resolution. Acta Crystallographica Section D Biological Crystallography. 59 (5), 835-842

Haynes, A., Maitlis, PM., Quyoum, R., Pulling, C., Adams, H., Spey, SE. and Strange, RW., (2002). Structure and reactivity of polymer-supported carbonylation catalysts. Journal of the Chemical Society, Dalton Transactions (12), 2565-2565

Ellis, MJ., Prudêncio, M., Dodd, FE., Strange, RW., Sawers, G., Eady, RR. and Hasnain, SS., (2002). Biochemical and crystallographic studies of the Met144Ala, Asp92Asn and His254Phe mutants of the nitrite reductase from Alcaligenes xylosoxidans provide insight into the enzyme mechanism. Journal of Molecular Biology. 316 (1), 51-64

Kuser, P., Hall, DR., Haw, ML., Neu, M., Evans, RW. and Lindley, PF., (2002). The mechanism of iron uptake by transferrins: the X-ray structures of the 18 kDa NII domain fragment of duck ovotransferrin and its nitrilotriacetate complex. Acta Crystallographica Section D Biological Crystallography. 58 (5), 777-783

Hasnain, SS., Murphy, LM., Strange, RW., Grossmann, JG., Clarke, AR., Jackson, GS. and Collinge, J., (2001). XAFS study of the high-affinity copper-binding site of human PrP 91–231 and its low-resolution structure in solution 1 1Edited by I. A. Wilson. Journal of Molecular Biology. 311 (3), 467-473

Ellis, MJ., Dodd, FE., Strange, RW., Prudêncio, M., Sawers, G., Eady, RR. and Hasnain, SS., (2001). X-ray structure of a blue copper nitrite reductase at high pH and in copper-free form at 1.9 Å resolution. Acta Crystallographica Section D Biological Crystallography. 57 (8), 1110-1118

Hough, MA., Strange, RW. and Hasnain, SS., (2000). Conformational variability of the Cu site in one subunit of bovine CuZn superoxide dismutase: the importance of mobility in the Glu119-Leu142 loop region for catalytic function. Journal of Molecular Biology. 304 (2), 231-241

Cheung, K-C., Strange, RW. and Hasnain, SS., (2000). 3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation–reduction process: an integrated approach combining EXAFS and crystallography. Acta Crystallographica Section D Biological Crystallography. 56 (6), 697-704

Meyer-klaucke, W. and Strange, RW., (2000). X-ray absorption spectroscopy in biology – present and future. Synchrotron Radiation News. 13 (5), 17-21

Strange, RW., Murphy, LM., Dodd, FE., Abraham, ZHL., Eady, RR., Smith, BE. and Hasnain, SS., (1999). Structural and kinetic evidence for an ordered mechanism of copper nitrite reductase. Journal of Molecular Biology. 287 (5), 1001-1009

Miyatake, H., Mukai, M., Adachi, S-I., Nakamura, H., Tamura, K., Iizuka, T., Shiro, Y., Strange, RW. and Hasnain, SS., (1999). Iron Coordination Structures of Oxygen Sensor FixL Characterized by Fe K-edge Extended X-ray Absorption Fine Structure and Resonance Raman Spectroscopy. Journal of Biological Chemistry. 274 (33), 23176-23184

Hall, JF., Kanbi, LD., Strange, RW. and Hasnain, SS., (1999). Role of the Axial Ligand in Type 1 Cu Centers Studied by Point Mutations of Met148 in Rusticyanin†. Biochemistry. 38 (39), 12675-12680

Cheung, K-C., Strange, R., Harvey, I., Dobson, B., Derbyshire, G., Kay, J., Binsted, N., Linford, R. and Hasnain, SS., (1999). Conversion of the oldest XAFS station at the first dedicated SR source to a state-of-the-art XAFS facility. Journal of Synchrotron Radiation. 6 (3), 161-163

Harvey, I., Strange, RW., Schneider, R., Gormal, CA., David Garner, C., Samar Hasnain, S., Richards, RL. and Smith, BE., (1998). X-ray absorption spectroscopic studies of the binding of ligands to FeMoco of nitrogenase from Klebsiella pneumoniae. Inorganica Chimica Acta. 275-276, 150-158

Grossmann, JG., Crawley, JB., Strange, RW., Patel, KJ., Murphy, LM., Neu, M., Evans, RW. and Hasnain, SS., (1998). The nature of ligand-induced conformational change in transferrin in solution. an investigation using X-ray scattering, XAFS and site-directed mutants. Journal of Molecular Biology. 279 (2), 461-472

Murphy, LM., Strange, RW. and Hasnain, SS., (1997). A critical assessment of the evidence from XAFS and crystallography for the breakage of the imidazolate bridge during catalysis in CuZn superoxide dismutase. Structure. 5 (3), 371-379

Eady, RR., Smith, BE., Abraham, ZHL., Dodd, FE., Grossmann, JG., Murphy, LM., Strange, RW. and Hasnain, SS., (1997). Difference EXAFS Studies of Nitrogenase and Nitrite Reductase Enzymes of the Nitrogen Cycle. Le Journal de Physique IV. 7 (C2)

Murphy, LM., Strange, RW. and Hasnain, SS., (1997). Does the Imidazole Bridge Break During Catalysis in Cu, Zn Superoxide Dismutase ? Judgement from XAFS and Crystallography. Le Journal de Physique IV. 7 (C2)

Strange, RW., Murphy, LM., Karlsson, BG., Reinhammar, B. and Hasnain, SS., (1996). Effect of pH and Ligand Binding on the Structure of the Cu Site of the Met121Glu Mutant of Azurin fromPseudomonas aeruginosa. Biochemistry. 35 (50), 16391-16398

Roach, PL., Clifton, IJ., Hensgens, CMH., Shibata, N., Long, AJ., Strange, RW., Hasnain, SS., Schofield, CJ., Baldwin, JE. and Hajdu, J., (1996). Anaerobic Crystallisation of an Isopenicillin N Synthase . Fe(II) . Substrate Complex Demonstrated by X-Ray Studies. European Journal of Biochemistry. 242 (3), 736-740

Strange, RW., Reinhammar, B., Murphy, LM. and Hasnain, SS., (1995). Structural and spectroscopic studies of the copper site of stellacyanin. Biochemistry. 34 (1), 220-231

Strange, RW., Dodd, FE., Abraham, ZHL., Grossmann, JG., Brüser, T., Eady, RR., Smith, BE. and Hasnain, SS., (1995). The substrate-binding site in Cu nitrite reductase and its similarity to Zn carbonic anhydrase. Nature Structural & Molecular Biology. 2 (4), 287-292

Murphy, LM., Dobson, BR., Neu, M., Ramsdale, CA., Strange, RW. and Hasnain, SS., (1995). Quick Fluorescence-EXAFS: an Improved Method for Collection of Conventional XAFS Data, an Improved Method for Collection of Conventional XAFS Data and for Studying Reaction Intermediates in Dilute Systems. Journal of Synchrotron Radiation. 2 (1), 64-69

Grossmann, JG., Strange, RW., Murphy, LM., Hasnain, SS., Haldane, SAT., Eady, RR., Eldridge, ME. and Smith, BE., (1995). X-ray structural studies in solution of component 1 proteins from nitrogenase. Journal of Inorganic Biochemistry. 59 (2-3), 338-338

Grossmann, JG., Strange, RW., Hasnain, SS., Harvey, I. and Ingledew, WJ., (1995). Modelling the Cu site in Rusticyanin-x-ray absorption studies and sequence homology to other copper proteins. Journal of Inorganic Biochemistry. 59 (2-3), 710-710

Grossmann, JG., Ingledew, WJ., Harvey, I., Strange, RW. and Hasnain, SS., (1995). X-ray absorption studies and homology modeling define the structural features that specify the nature of the copper site in rusticyanin. Biochemistry. 34 (26), 8406-8414

Strange, RW., Murphy, L., Karlsson, BG., Lindley, PF., Lundberg, LG., Reinhammar, B. and Hasnain, SS., (1994). Crystallization and preliminary crystallographic data for the azurin mutant End-121 fromPseudomonas aeruginosa. Acta Crystallographica Section D Biological Crystallography. 50 (1), 37-39

Strange, R., Morante, S., Stefanini, S., Chiancone, E. and Desideri, A., (1993). Nucleation of the iron core occurs at the three-fold channels of horse spleen apoferritin: an EXAFS study on the native and chemically-modified protein. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1164 (3), 331-334

Sanyal, I., Karlin, KD., Strange, RW. and Blackburn, NJ., (1993). Chemistry and structural studies on the dioxygen-binding copper-1,2-dimethylimidazole system. Journal of the American Chemical Society. 115 (24), 11259-11270

Murphy, LM., Strange, RW., Karlsson, BG., Lundberg, LG., Pascher, T., Reinhammar, B. and Hasnain, SS., (1993). Structural characterization of azurin from Pseudomonas aeruginosa and some of its methionine-121 mutants. Biochemistry. 32 (8), 1965-1975

Murphy, LM., Strange, RW., Karlsson, BG., Lundberg, LG., Pascher, T., Reinhammar, B. and Hasnain, SS., (1993). Structural Characterisation of Azurin fromPseudomonas aeruginosaand some of Its Methionine-121 Mutants. Japanese Journal of Applied Physics. 32 (S2), 561-561

MacLachlan, DJ., Hallahan, BJ., Ruffle, SV., Nugent, JHA., Evans, MCW., Strange, RW. and Hasnain, SS., (1992). An e.x.a.f.s. study of the manganese O2-evolving complex in purified Photosystem II membrane fractions. The S1 and S2 states. Biochemical Journal. 285 (2), 569-576

Blackburn, NJ., Strange, RW., Carr, RT. and Benkovic, SJ., (1992). X-ray absorption studies of the copper-dependent phenylalanine hydroxylase from Chromobacterium violaceum. Comparison of the copper coordination in oxidized and dithionite-reduced enzymes. Biochemistry. 31 (23), 5298-5303

Binsted, N., Strange, RW. and Hasnain, SS., (1992). Constrained and restrained refinement in EXAFS data analysis with curved wave theory. Biochemistry. 31 (48), 12117-12125

Berry, FJ., Greaves, C., Lobo, RC. and Strange, R., (1992). Structural implications of oxygen stoichiometry in Eu1.3Sr1.7Cu2O6-δ. Journal of Alloys and Compounds. 189 (2), 235-240

Berry, RJ., Greaves, C., Lobo, RC. and Strange, R., (1992). The local coordination of copper in Sr2CuO3 and Sr2CuO4−xx ∼ 0.1: A study by extended x-ray absorption fine structure. Polyhedron. 11 (18), 2331-2333

Sanyal, I., Strange, RW., Blackburn, NJ. and Karlin, KD., (1991). Formation of a copper-dioxygen complex (Cu2-O2) using simple imidazole ligands. Journal of the American Chemical Society. 113 (12), 4692-4693

Pettingill,, TM., Strange, RW. and Blackburn, NJ., (1991). Carbonmonoxy dopamine β-hydroxylase. Structural characterization by Fourier transform infrared, fluorescence, and x-ray absorption spectroscopy. Journal of Biological Chemistry. 266 (26), 16996-17003

Blackburn, NJ., Hasnain, SS., Pettingiill, TM. and Strange, RW., (1991). Copper K-extended x-ray absorption fine structure studies of oxidized and reduced dopamine beta-hydroxylase. Confirmation of a sulfur ligand to copper(I) in the reduced enzyme. Journal of Biological Chemistry. 266 (34), 23120-23127

Berry, FJ., Greaves, C., Lobo, RC. and Strange, R., (1991). Lanthanum L3-, europium L3- and copper K-edge EXAFS investigation of lanthanum copper oxides. Journal of the Chemical Society, Faraday Transactions. 87 (9), 1405-1405

Strange, RW., Alagna, L., Durham, P. and Hasnain, SS., (1990). An understanding of the x-ray absorption near-edge structure of copper(II) imidazole complexes. Journal of the American Chemical Society. 112 (11), 4265-4268

Berry, FJ., Jobson, S., Liwu, L., Strange, R. and Changhai, X., (1990). An iron-57 Mössbauer spectroscopy and EXAFS study of the hydrogen pretreatment of titania-supported iron-ruthenium catalysts. Hyperfine Interactions. 57 (1-4), 1753-1758

Battle, PD., Jones, CW., Lightfoot, P. and Strange, R., (1990). High-pressure phase transitions in the 6H perovskitesBa3MSb2O9(M =Mg, Ni, Zn). Journal of Solid State Chemistry. 85 (1), 144-150

Navaratnam, S., Myles, GM., Strange, RW. and Sancar, A., (1989). Evidence from extended X-ray absorption fine structure and site-specific mutagenesis for zinc fingers in UvrA protein of Escherichia coli. Journal of Biological Chemistry. 264 (27), 16067-16071

Knowles, PF., Strange, RW., Blackburn, NJ. and Hasnain, SS., (1989). EXAFS studies on pig plasma amine oxidase. A detailed structural analysis using the curved wave multiple scattering approach. Journal of the American Chemical Society. 111 (1), 102-107

Blackburn, NJ., Strange, RW., Reedijk, J., Volbeda, A., Farooq, A., Karlin, KD. and Zubieta, J., (1989). X-ray absorption edge spectroscopy of copper(I) complexes. Coordination geometry of copper(I) in the reduced forms of copper proteins and their derivatives with carbon monoxide. Inorganic Chemistry. 28 (7), 1349-1357

Berry, FJ., Changhai, X., Jobson, S. and Strange, R., (1989). EXAFS investigation of structural changes induces during the pretreatment of a titania-supported iron–ruthenium catalyst. Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases. 85 (11), 3891-3891

Battle, PD., Gibb, TC. and Strange, R., (1989). A study of a new incommensurate phase in the system SrMn1−xCoxO3−y. Journal of Solid State Chemistry. 81 (2), 217-229

Karlin, KD., Ghosh, P., Cruse, RW., Farooq, A., Gultneh, Y., Jacobson, RR., Blackburn, NJ., Strange, RW. and Zubieta, J., (1988). Dioxygen-copper reactivity: generation, characterization, and reactivity of a hydroperoxodicopper(II) complex. Journal of the American Chemical Society. 110 (20), 6769-6780

Blackburn, NJ., Strange, RW., Farooq, A., Haka, MS. and Karlin, KD., (1988). X-ray absorption studies of three-coordinate dicopper(I) complexes and their dioxygen adducts. Journal of the American Chemical Society. 110 (13), 4263-4272

Strange, RW., Blackburn, NJ., Knowles, PF. and Hasnain, SS., (1987). X-ray absorption spectroscopy of metal-histidine coordination in metalloproteins. Exact simulation of the EXAFS of tetrabis(imidazole)copper(II) nitrate and other copper-imidazole complexes by the use of a multiple-scattering treatment. Journal of the American Chemical Society. 109 (23), 7157-7162

Blackburn, NJ., Strange, RW., McFadden, LM. and Hasnain, SS., (1987). Anion binding to bovine erythrocyte superoxide dismutase studied by x-ray absorption spectroscopy. A detailed structural analysis of the native enzyme and the azido and cyano derivatives using a multiple-scattering approach. Journal of the American Chemical Society. 109 (23), 7162-7170

Blackburn, NJ., Strange, RW., Cruse, RW. and Karlin, KD., (1987). Dioxygen-copper reactivity: EXAFS studies of a peroxo-dicopper(II) complex. Journal of the American Chemical Society. 109 (4), 1235-1237

Book chapters (1)

Strange, RW. and Hasnain, SS., (2005). Combined Use of XAFS and Crystallography for Studying Protein–Ligand Interactions in Metalloproteins. In: Protein-Ligand Interactions. Humana Press. 167- 196. 1592599125

Conferences (8)

Bielecki, M., Antonyuk, S., Strange, RW., Smalley, JW., Smiga, M., Stepien, P., Mackiewicz, P., Nocen, P., Olczak, M. and Olczak, T., (2018). Prevotella intermedia produces two homologous proteins to Porphyromonas gingivalis HmuY with different heme-binding properties

Bielecki, M., Antonyuk, S., Strange, RW., Smalley, JW., Smiga, M., Stepien, P., Mackiewicz, P., Nocen, P., Olczak, M. and Olczak, T., (2018). Porphyromonas gingivalis HmuY and Tannerella forsythia Tfo - two homologous proteins with different heme-binding properties

Horrell, S., Kekilli, D., Sen, K., Yong, CW., Keal, TW., Hough, MA. and Strange, RW., (2017). Multiple structures from one crystal serial crystallography (MSOX): variable temperature movies

Sen, K., Hough, MA., Yong, CW., Keal, TW. and Strange, RW., (2017). Solvent accessibility and ligand binding in AcNiR, a two domain copper nitrite reductase

Hough, MA., Kekilli, D., Horrell, S., Sen, K., Yong, C., Keal, TWK., Antonyuk, SV., Eady, RR., Hasnain, SS. and Strange, RW., (2017). MSOX crystallography and simulations to capture redox enzyme catalysis

Hough, M., Kekilli, D., Horrell, S., Dworkowski, FSN., Owen, RL., Antonyuk, SV., Eady, RR., Hasnain, S. and Strange, RW., (2016). Single-crystal serial crystallography to capture redox enzyme catalysis and dynamics

Hough, MA., Kekilli, D., Horrell, S., Sen, K., Yong, C., Keal, TWK., Antonyuk, SV., Eady, RR., Hasnain, SS. and Strange, RW., (2014). MSOX crystallography and simulations to capture redox enzyme catalysis

Binsted, N., Strange, RW. and Hasnain, SS., (1993). Constrained and Restrained Refinement in EXAFS Data Analysis with Curved Wave Theory

Reports and Papers (1)

Murphy, LM., Strange, RW., Karlsson, BG., Lundberg, LG., Pascher, T., Reinhammar, B. and Hasnam, SS., (1993). Structural characterization of Azurin from Pseudomonas aeruginosa and some of its methionine-121 mutants

Grants and funding

2020

Replacement EPR spectrometer to the multiuser Biomedical EPR Facility at the University of Essex

Biotechnology and Biological Sciences Research Council

2018

High hit-rate, high-throughput serial protein crystallography at SACLA using fixed target silicon nitride chips

Biotechnology and Biological Sciences Research Council

2017

Transition metal controlled nitrogen chemistry in zeolite and protein environments using a unified quantum embedding model

Engineering and Physical Sciences Research Council

2015

Dynamics of Electron and Proton Transfer Chemistry and Hybrid Copper-Haem Enzymes

Biotechnology & Biology Science Res.Council

2014

Enzyme Catalysis in Action: 3D Movies of X-ray Induced Chemical Reactions in Protein Crystals

Leverhulme Trust

Contact

rstrange@essex.ac.uk

Location:

Colchester Campus

Academic support hours:

Final Year Project students (3) Protein Bioinformatics (2 lectures, 3 Workshops) MSc research Project student PhD student