Use microarray analysis to study the changes in gene expression occurring under light/dark transitions and in response to CO₂ fluctuations (cultures grown using the chemostat facilities at Essex will be used to prepare RNA for microarray analysis at San Marcos).

Employ chemostat culture technology to evaluate inorganic carbon acquisition pathways utilized byE. E.huxleyi in response to CO₂ and trace metal (Zn, Co and Cd) fluctuations.

Identify the effect of the environmental fluctuations (mentioned above) on the host-virus interaction, both at the cellular (culture characteristics) and genomic level (microarrays).

Identify the cellular location of candidate biomineralization proteins encoded by gene sequences that are differentially expressed in calcifying E. huxleyi cells as compared to non-calcifying cells.

Develop transfection protocols for GTP tagged constructs of genes of interest in E. huxleyi cells.